Assessment of the clinical significance of antigenic and functional levels of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinomas
Assessment of the clinical significance of antigenic and functional levels of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinomas
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DOI：
10.1016/j.clinbiochem.2012.07.099
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发表时间：
2012-11-01
影响因子：
2.8
通讯作者：
Chahed, Karim
中科院分区：
文献类型：
--
作者：
ben Anes, Amel;ben Nasr, Hela;Chahed, Karim
Objectives: To determine the clinical significance of alpha 1-proteinase inhibitor (alpha 1-Pi) in infiltrating ductal breast carcinoma patients.Design and methods: Serum levels of alpha 1-Pi, tryptic specific inhibitory capacity and alpha 1-Pi circulating immune complexes were determined using radial immunodiffusion, BAPNA assays and ELISA, respectively. 2-DE-MS and immunohistochemistry were performed to examine alpha 1-Pi protein expression.Results: A decreased serum level of alpha 1-Pi was found among breast cancer patients in comparison to controls. In addition, we found a significantly decreased mean level of alpha 1-Pi in the node metastatic group when compared to node negative patients. However, the functional activity of the inhibitor did not decrease proportionately. Through 2-DE analyses, a differential expression of alpha 1-Pi isoforms according to tumor stage and node metastatic development was found.Conclusions: Both alpha 1-Pi levels and specific activity could be a source of complementary clinical information and may provide useful information for a better understanding of the mechanisms of metastasis. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
