Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody
Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody
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使用沙眼-性病淋巴肉芽肿（沙眼衣原体）抗原进行简化的微量免疫荧光试验，用作抗体筛选试验
DOI：
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发表时间：
1975
影响因子：
9.4
通讯作者：
K K Holmes
中科院分区：
文献类型：
--
作者：
S. Wang;J. Grayston;E R Alexander;K K Holmes
摘要设计了一种简单的沙眼淋巴肉芽肿性病（沙眼衣原体）抗原微免疫荧光试验，作为人血清抗体的筛选试验。该测试与我们的标准程序不同，它将15种不同的免疫类型合并到9个抗原池中，只使用三种血清稀释剂，并且不使用重复的载玻片。对于一个给定的工作单位，筛选测试可以在至少六倍于标准测试的血清上进行。结果表明，该方法的灵敏度与标准方法相当，根据抗体模式确定特定免疫型的能力（72%）仅略低于标准方法（84%）。筛查试验对来自西雅图地区不同人群的876名患者进行。试验表明，抗体经常出现在参加性病诊所的人群中（60%），而在没有性病的成年人群体中（25%）普遍存在。9%的15岁以下儿童检测出抗体；这一发现的意义尚不清楚。
A simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens has been devised as a screening test for antibody in human sera. The test differs from our standard procedure by amalgamating 15 different immunotypes into nine antigen pools, by using only three serum dilutions, and by dropping use of duplicate slides. The screening test could be performed on at least six times as many sera as the standard test for a given unit of effort. It was shown to have a sensitivity equal to the standard test and an ability to determine specific immunotype on the basis of antibody pattern (72%) only slightly reduced from the standard test (84%). The screening test was carried out on 876 patients from different population groups in the Seattle area. The tests showed that antibody was present frequently in persons attending venereal disease clinics (60%) and commonly (25%) in a group of adults without venereal disease. Nine percent of children under 15 years of age tested had antibody; the significance of this finding is unknown.