A differential array of metalated synthetic receptors for the analysis of tripeptide mixtures.
A differential array of metalated synthetic receptors for the analysis of tripeptide mixtures.
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DOI：
10.1021/ja055696g
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发表时间：
2005-11
影响因子：
15
通讯作者：
Aaron T. Wright;E. Anslyn;J. McDevitt
中科院分区：
文献类型：
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作者：
Aaron T. Wright;E. Anslyn;J. McDevitt
本文报道了一种新的用于识别和区分三肽和三肽混合物的交叉反应差异阵列内的树脂结合受体库。使用主成分分析的模式识别显示四个类似的三肽和三个三肽混合物的完全歧视。该文库由Cu（II）为中心的核心和两个近端附加的向外发出的三肽臂组成。使用组合化学制备一个三肽臂以产生文库的差异性质。从文库中随机选择30个树脂结合的受体，并将其置于包括集成微流体元件的硅微芯片阵列中，并使用指示剂摄取测定法进行比色信号传导。指示剂橙子G产生受体和分析物之间的关联程度的准确测量，如通过指示剂摄取测定的动力学分析所确定的。在本文中，我们详细介绍了使用一种新的受体库用于差分传感的方法。这项工作进一步证明了合成受体的差分阵列用于识别和区分复杂生物分析物的能力和实用性。
A novel library of resin-bound receptors within a cross-reactive differential array for the identification and discrimination of tripeptides and tripeptide mixtures is reported. Pattern recognition using principal component analysis showed complete discrimination of four similar tripeptides and three tripeptide mixtures. The library is comprised of a Cu(II)-centered core with two proximally appended tripeptide arms emanating outward. One tripeptide arm was prepared using combinatorial chemistry to generate the differential nature of the library. Thirty resin-bound receptors were randomly selected from the library and placed within a silicon microchip array that included integrated microfluidics elements, and an indicator-uptake assay was used for colorimetric signaling. The indicator Orange G yielded an accurate measure of the degree of association between receptors and analytes as determined by kinetic analysis of the indicator-uptake assays. Within this paper we detail the method used for differential sensing using a novel receptor library. This work further demonstrates the power and utility of a differential array of synthetic receptors for identification and discrimination of complex bioanalytes.