Lectin-induced enhancement of superoxide anion production by red tide phytoplankton
Lectin-induced enhancement of superoxide anion production by red tide phytoplankton
复制标题
凝集素诱导的赤潮浮游植物超氧阴离子产生增强
DOI：
10.1007/s002270050331
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发表时间：
1998
期刊：
影响因子：
2.4
通讯作者：
T. Muramatsu
中科院分区：
文献类型：
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作者：
T. Oda;A. Nakamura;T. Okamoto;A. Ishimatsu;T. Muramatsu
Chattonella marina 是一种针藻类鞭毛虫，是一种剧毒赤潮浮游植物，对养鱼业造成严重损害。最近的研究表明，Chattonellaspp。在活着的时候不断释放超氧阴离子（O2−）。赤潮异形藻（Heterosigma akashiwo）是另一种针藻类鞭毛虫，也会产生 O2−。在本研究中，我们发现伴刀豆球蛋白 A (Con A)、麦芽凝集素 (WGA) 和蓖麻子血凝素 (CBH) 等凝集素可刺激 C.码头和H. akashiwoto 以浓度依赖性方式产生大量的 O2−。凝集素特异性糖有效抑制凝集素诱导的 O2−产生增加，表明凝集素的作用主要是通过这些凝集素与鞭毛细胞表面存在的碳水化合物部分的相互作用来介导的。与天然 Con A（四价）的强大能力相比，琥珀酰化 Con A（二价）对这些鞭毛虫仅表现出轻微的刺激作用。用蛋白酶 K 处理 30 分钟完全抑制 O2−产生，而不影响鞭毛虫的活力。这些结果表明细胞表面氧化还原酶可能参与 O2−产生，并且此类酶负责凝集素刺激。
Chattonella marina, a raphidophycean flagellate, is a highly toxic red tide phytoplankton which causes severe damage to fish farming. Recent studies demonstrated thatChattonellaspp. continuously release superoxide anions (O2−) while they are living.Heterosigma akashiwo, another raphidophycean flagellate, also produces O2−. In the present study, we found that lectins such as concanavalin A (Con A), wheat germ agglutinin (WGA), and castor bean hemagglutinin (CBH) stimulatedC. marinaandH. akashiwoto generate enhanced amounts of O2−in a concentration-dependent manner. The lectin-specific sugars potently inhibited the lectin-induced increase of O2−production, suggesting that the effects of lectins are mediated mainly through the interaction of these lectins with carbohydrate moiety present on the flagellate cell surface. In contrast to the potent ability of native Con A (tetravalent), succinylated Con A (divalent) showed only a slight stimulative effect on these flagellates. O2−production was totally inhibited by treatment with proteinase K for 30 min, without affecting the viabilities of flagellates. These results suggest that cell-surface redox enzymes may be involved in O2−production, and such enzymes are responsible for the lectin-stimulation.