DISTRIBUTION AND MORPHOLOGICAL-CHARACTERISTICS OF OLIGODENDROCYTES IN THE RAT HIPPOCAMPUS IN-SITU AND IN-VITRO - AN IMMUNOCYTOCHEMICAL STUDY WITH THE MONOCLONAL RIP ANTIBODY
DISTRIBUTION AND MORPHOLOGICAL-CHARACTERISTICS OF OLIGODENDROCYTES IN THE RAT HIPPOCAMPUS IN-SITU AND IN-VITRO - AN IMMUNOCYTOCHEMICAL STUDY WITH THE MONOCLONAL RIP ANTIBODY
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DOI：
10.1007/bf01189817
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
FROTSCHER, M
中科院分区：
文献类型：
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作者：
BERGER, T;FROTSCHER, M
用Rip单克隆抗体，通过光镜和电镜免疫细胞化学研究了大鼠海马原位和器官型切片培养中的少突胶质细胞。我们的研究结果证实，这种抗体只染色少突胶质细胞，而星形胶质细胞和神经元没有标记。在光镜下，免疫阳性细胞的髓鞘少突胶质细胞的外观与其特征性的管状突起。电镜下，染色细胞与髓鞘紧密接触，但与内皮细胞的基底层不紧密接触。RIP阳性的少突胶质细胞在成年大鼠海马结构中分布不均匀。在一般情况下，他们丰富的层已知含有许多传入和传出纤维。在海马适当的，有一个特别强的免疫标记的辐射层领域CA 2。在齿状筋膜中，门区显示高密度的细胞，尤其是在颗粒细胞层附近。在幼龄动物（15-18日龄）中发现了类似的免疫阳性细胞分布;然而，标记细胞的密度较低，特别是在门部。海马切片培养的免疫标记细胞显示出髓鞘少突胶质细胞的特征。此外，他们表现出器官型分布，虽然传入和传出纤维的预测通常由这些细胞髓鞘在这些条件下缺席。
Oligodendrocytes in the rat hippocampus in situ and in organotypic slice cultures were studied by light and electron microscopic immunocytochemistry using the monoclonal Rip antibody. Our results confirm that this antibody exclusively stains oligodendrocytes, while astrocytes and neurons are not labelled. In the light microscope, immunopositive cells had the appearance of myelinating oligodendrocytes with their characteristic tubular processes. In the electron microscope, stained cells showed intimate contacts with myelin sheaths but not with the basal laminae of endothelial cells. Rip-positive oligodendrocytes were unevenly distributed in the adult rat hippocampal formation. In general, they were abundant in layers known to contain many afferent and efferent fibres. In the hippocampus proper, there was a particularly strong immunolabelling of stratum radiatum of field CA2. In the fascia dentata, the hilar region displayed a high cell density, especially in the vicinity of the granule cell layer. A similar distribution of immunopositive cells was found in young animals (15-18 days old); however, the density of labelled cells was lower, particularly in the hilus. Immunolabelled cells in slice cultures of hippocampus displayed the characteristics of myelinating oligodendrocytes. Moreover, they showed an organotypic distribution, although afferent and efferent fibre projections normally myelinated by these cells were absent under these conditions.