Porphyromonas gingivalis strain variability and periodontitis
Porphyromonas gingivalis strain variability and periodontitis
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DOI：
10.1128/jcm.37.12.4028-4033.1999
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发表时间：
1999-12-01
影响因子：
9.4
通讯作者：
Leys, EJ
中科院分区：
文献类型：
--
作者：
Griffen, AL;Lyons, SR;Leys, EJ
为了确定牙龈卟啉单胞菌菌株在人类牙周炎中的毒力是否存在差异，研究了它们在一组有明确牙周炎指标的受试者和一组健康的年龄匹配的对照组中的分布。用基于聚合酶链式反应的方法确定两组中存在异源双链型牙龈假单胞菌。该方法依赖于核糖体内间隔区(ISR)的多态检测，用P，牙周炎特异的聚合酶链式反应产生的ISR片段与参比菌株的片段杂交，并用聚丙烯酰胺凝胶电泳法观察不同序列杂交后形成的异源双链。通过与一组参考菌株的比较，特征指纹可以识别临床样本中的异源双链类型。共抽取130例成人牙周炎患者和181例正常对照。通过这种方法，在该人群中检测到11种异源双重型牙龈假单胞菌。有足够的数字可供对其中六种类型进行统计分析。异源双链hW83型与牙周炎密切相关(P=0.0000)，另外两种类型h49417和hHG1691也与牙周炎显著相关。其余h23A4、h381和hA7A1基因在牙周炎患者中的检出频率高于正常人，但差异无统计学意义。这些数据表明，人类牙周炎的毒力在不同的牙龈假单胞菌菌株之间存在差异，它们识别出一个明显的高毒力亚群。
To determine if there is variability in virulence among strains of Porphyromonas gingivalis in human periodontitis, their distribution in a group of subjects with clear indicators of periodontitis and in a healthy, age-matched control group was examined. The presence of heteroduplex types of P. gingivalis in the two groups was determined with a PCR-based assay. This assay relied on detection of polymorphisms in the ribosomal internal spacer region (ISR), ISR fragments generated by PCR with P, gingivalis-specific primers were hybridized to fragments from reference strains, and the formation of heteroduplexes from the hybridization of nonidentical sequences was observed by polyacrylamide gel electrophoresis. Characteristic fingerprints from comparison with a panel of reference strains allowed the identification of heteroduplex types in clinical samples. One hundred thirty adults with periodontitis and 181 controls were sampled. With this approach, 11 heteroduplex types of P, gingivalis were detected in the population. Sufficient numbers were available for statistical analysis of six of these types. Heteroduplex type hW83 was found to be very strongly associated with periodontitis (P = 0.0000), and two additional types, h49417 and hHG1691, were also significantly associated with disease. The remaining types, h23A4, h381, and hA7A1, were detected more frequently in subjects with periodontitis than in healthy subjects, but the difference was not significant. These data indicate that virulence in human periodontitis varies among strains of P, gingivalis, and they identify an apparently highly virulent subgroup.