Fourier transform-second-harmonic generation imaging of biological tissues
Fourier transform-second-harmonic generation imaging of biological tissues
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DOI：
10.1364/oe.17.014534
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发表时间：
2009-08-17
期刊：
影响因子：
3.8
通讯作者：
Toussaint, Kimani C., Jr.
中科院分区：
文献类型：
--
作者：
Rao, Raghu Ambekar Ramachandra;Mehta, Monal R.;Toussaint, Kimani C., Jr.
采用傅里叶变换-二次谐波成像技术获得生物组织中胶原纤维的定量指标。特别是，确定了猪气管，耳朵和角膜中感兴趣的选定区域胶原纤维的首选方向和最大空间频率。这些指标在应用于耳中的胶原纤维时保持一致，这可以从观察中得到预期。气管内的胶原纤维具有较大的方向性标准差，最大空间频率变化较大。此外，这些指标还用于通过角膜的3D堆栈来研究结构变化。这项技术可以作为定量标记来评估胶原纤维的结构，胶原纤维可能因疾病或物理损伤而改变。(C) 2009年美国光学学会
Fourier transform-second-harmonic generation imaging is employed to obtain quantitative metrics of collagen fibers in biological tissues. In particular, the preferred orientation and maximum spatial frequency of collagen fibers for selected regions of interest in porcine trachea, ear, and cornea are determined. These metrics remain consistent when applied to collagen fibers in the ear, which can be expected from observation. Collagen fibers in the trachea are more random with large standard deviations in orientation, and large variations in maximum spatial frequency. In addition, these metrics are used to investigate structural changes through a 3D stack of the cornea. This technique can be used as a quantitative marker to assess the structure of collagen fibers that may change due to damage from disease or physical injury. (C) 2009 Optical Society of America