A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species
A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species
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DOI：
10.1016/j.mimet.2004.11.016
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发表时间：
2005-05-01
影响因子：
2.2
通讯作者：
Yamaguchi, H
中科院分区：
文献类型：
--
作者：
Mirhendi, H;Makimura, K;Yamaguchi, H
以26 SrDNA为靶点，采用CfoI和BstF 51两种限制性内切酶，建立了一种用于鉴定马拉色菌属11种的PCR-RFLP方法。本研究不仅成功地鉴定了标准株和模式株，还鉴定了13株临床分离株。鉴定结果经DNA测序证实。(C)2004 Elsevier B. V.保留所有权利。
A PCR-RFLP method targeted toward 26S rDNA and with 2 restriction enzymes, CfoI and BstF51, was developed to identify 11 Malassezia species. Not only type and standard strains but also 13 clinical isolates were identified successfully in this study. The results of identifications were confirmed by DNA sequencing. (C) 2004 Elsevier B.V. All rights reserved.