Enzymatic synthesis and purification of L-pyrroline-5-carboxylic acid.
Enzymatic synthesis and purification of L-pyrroline-5-carboxylic acid.
复制标题
L-吡咯啉-5-羧酸的酶法合成和纯化。
DOI：
10.1016/0003-2697(77)90145-2
复制
发表时间：
1977
影响因子：
2.9
通讯作者：
J. Phang
中科院分区：
文献类型：
--
作者：
R. Smith;S. Downing;J. Phang
Δ1-Pyrroline-5-carboxylic acid (P5C) is an intermediate in the metabolism of proline, ornithine, and glutamic acid. It has been obtainable by chemical synthesis only as a mixture of the d- and l-stereoisomers. We report a method for the enzymatic synthesis of P5C on a preparative scale. The P5C is formed from l-ornithine by purified ornithine aminotransferase and then isolated by Dowex-50W cation-exchange resin chromatography. The purified compound exists entirely as the biologically active l-stereoisomer. With [14C]ornithine as precursor, high specific activity uniformly labeled [14C]P5C can be obtained.
