Lipid droplets in stress protection: distinct mechanisms of lipid droplet microautophagy.
Lipid droplets in stress protection: distinct mechanisms of lipid droplet microautophagy.
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应激保护中的脂滴：脂滴微自噬的独特机制。
DOI：
10.1080/27694127.2022.2067643
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Pon,LizaA
中科院分区：
文献类型：
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作者：
Liao,Pin-Chao;Pon,LizaA
脂滴（LD）是作为脂质储存场所的细胞器。LD也参与了对蛋白毒性或脂毒性应激的细胞反应，作为螯合功能障碍或过量蛋白质或脂质的位点，并靶向这些货物，以通过LD微自噬（microlipophagy，µLP）降解。在这里，我们描述了酵母中µLP的两种机制，它们是由不同的应激源触发的。µLP发生在暴露于严重营养限制的酵母中的液泡膜中的筏状液体有序微区。相比之下，在酵母暴露于ER应激或不太严重的营养限制，LD在液泡的摄取是液体有序（Lo）的微结构域独立的，并依赖于液泡膜重塑所需的内体分选复合物的运输（ESCRT）介导的。
Lipid droplets (LDs) are organelles that function as sites for lipid storage. LDs have also been implicated in the cellular response to proteotoxic or lipotoxic stress as sites for sequestering dysfunctional or excess proteins or lipids, and targeting those cargos for degradation by LD microautophagy (microlipophagy, µLP). Here, we describe two mechanisms for µLP in yeast, which are triggered by different stressors. µLP occurs at raft-like liquid ordered microdomains in the vacuolar membrane in yeast exposed to severe nutrient limitations. In contrast, in yeast exposed to ER stress or less severe nutrient limitations, LD uptake at the vacuole is liquid ordered (Lo) microdomain-independent and dependent upon vacuolar membrane remodeling mediated by endosomal sorting complexes required for transport (ESCRT).