Alveolar macrophages in sarcoidosis coexpress high levels of CD86 (B7.2), CD40, and CD30L
Alveolar macrophages in sarcoidosis coexpress high levels of CD86 (B7.2), CD40, and CD30L
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DOI：
10.1165/ajrcmb.17.1.2781
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发表时间：
1997-07-01
影响因子：
6.4
通讯作者：
Isler, P
中科院分区：
文献类型：
--
作者：
Nicod, LP;Isler, P
来自结节病患者的肺泡巨噬细胞(AM)被证明是良好的抗原提呈细胞(APC)，而正常AM通常无效。我们在连续10例结节病患者中证实，他们的大多数AM与正常AM不同，确实共表达高水平的CD86、CD40和CD30L，所有这些都是已知对T细胞激活重要的。CD80在结节型AM上的表达也略高于正常AM，但仅在结节型AM的26+/-6%(平均+/-SEM)上检测到结节AM表达CD86和CD40或CD86和CD30L的百分比之间有很好的相关性。然而，CD80和CD86阳性AM在这些患者中的百分比之间没有相关性。CD86封闭抗体可使结节患者AM诱导的同种异体T细胞增殖减少80%以上。这项研究提供的证据表明，在结节病等病理状态下，AM可以表达CD86等T细胞激活的功能性共刺激分子，CD86被认为是更专业的APC(如树突状细胞)所特有的。
Alveolar macrophages (AM) from sarcoid patients have been shown to be good antigen presenting cells (APC) unlike normal AM which are usually ineffective. We demonstrate in ten consecutive sarcoid patients that most of their AM, unlike normal AM, do coexpress high levels of CD86, CD40, and CD30L, all known to be important for T-cell activation. CD80 is also slightly more expressed on sarcoid AM than on normal AM, but is detected on only 26 +/- 6% (mean +/- SEM) of sarcoid AM. A good correlation is present between the percentage of sarcoid AM expressing CD86 and CD40 or CD86 and CD30L. However, no correlation is found between the percentage of CD80 and CD86 positive AM in these same patients. Blocking antibodies against CD86 were able to reduce by more than 80% allogeneic T-cell proliferation induced by the AM of sarcoid patients. This study provides evidence that AM can, in pathologic states such as sarcoidosis, express functional costimulatory molecules for T-cell activation such as CD86, thought to be rather specific for more professional APC such as dendritic cells.