Depot-Specific Expression of Lipolytic Genes in Human Adipose Tissues - Association Among CES1 Expression, Triglyceride Lipase Activity and Adiposity
Depot-Specific Expression of Lipolytic Genes in Human Adipose Tissues - Association Among CES1 Expression, Triglyceride Lipase Activity and Adiposity
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DOI：
10.5551/jat.6478
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发表时间：
2011-01-01
影响因子：
4.4
通讯作者：
Ishibashi, Shun
中科院分区：
文献类型：
--
作者：
Nagashima, Shuichi;Yagyu, Hiroaki;Ishibashi, Shun
目的：脂肪细胞脂解是由甘油三酯（TG）脂肪酶家族介导的，该家族包括脂肪敏感脂肪酶（LIPE）、脂肪甘油三酯脂肪酶（PNPLA 2）和羧酸酯酶1（CES 1）;然而，关于每个基因在不同库中的表达与TG脂肪酶活性或肥胖之间的关系知之甚少。我们测量了脂肪分解酶（LIPE，PNPLA 2和CES 1）的mRNA表达水平和TG脂肪酶活性的活检样品从皮下，网膜和肠系膜脂肪组织的34例接受腹部手术。结果与临床参数：肥胖的措施，参数胰岛素抵抗和血脂level.Results：PNPLA 2 mRNA水平略高于网膜脂肪比皮下脂肪。细胞内TG脂肪酶活性与皮下脂肪和肠系膜脂肪中CES 1的mRNA水平呈正相关，而与网膜脂肪中PNPLA 2的mRNA水平呈正相关。LIPE的mRNA水平与皮下脂肪中的各种肥胖指标呈负相关。CES 1的mRNA水平与肥胖的各种指标呈正相关，特别是在三个仓库中通过CT估计的那些;它们也与网膜脂肪中的血浆LDL-胆固醇水平呈正相关。与此相反，PNPLA 2的mRNA水平与adiposity.Conclusions：CES 1的表达与胞质TG脂肪酶活性以及与肥胖的正相关性表明，CES 1参与脂解，从而有助于肥胖相关表型的发展。另一方面，LIPE的表达与肥胖呈负相关。这些不同的脂肪分解基因的调控模式可能是与人类肥胖相关的复杂表型的基础。
Aim: Adipocyte lipolysis is mediated by a family of triglyceride (TG) lipases consisting of hormone-sensitive lipase (LIPE), adipose triglyceride lipase (PNPLA2) and carboxylesterase 1 (CES1); however, little is known about the relationship between the expression of each gene in different depots and TG lipase activity or obesityMethod: We measured both mRNA expression levels of the lipolytic enzymes (LIPE, PNPLA2 and CES1) and TG lipase activities of biopsy samples obtained from subcutaneous, omental and mesenteric adipose tissues of 34 patients who underwent abdominal surgery. The results were correlated with clinical parameters: adiposity measures, parameters for insulin resistance and plasma lipid levels.Results: PNPLA2 mRNA levels were slightly higher in omental fat than subcutaneous fat. Cytosolic TG lipase activities were positively correlated with the mRNA levels of CES1 in subcutaneous fat and mesenteric fat, while they were correlated with those of PNPLA2 in omental fat. The mRNA levels of LIPE were negatively correlated with various measures of adiposity in subcutaneous fat. The mRNA levels of CES1 were positively correlated with various measures of adiposity, particularly those estimated by CT in the three depots; they were also positively correlated with plasma LDL-cholesterol levels in omental fat. In contrast, the mRNA levels of PNPLA2 were not significantly associated with adiposity.Conclusions: The positive correlations of the expression of CES1 with cytosolic TG lipase activities as well as with adiposity suggest that CES1 is involved in lipolysis, thereby contributing to the development of obesity-associated phenotypes. On the other hand, the expression of LIPE is negatively correlated with adiposity. These distinct regulatory patterns of lipolytic genes may underlie the complex phenotypes associated with human obesity.