Immobilization of Biomolecules on the Surface of Electrospun Polycaprolactone Fibrous Scaffolds for Tissue Engineering
Immobilization of Biomolecules on the Surface of Electrospun Polycaprolactone Fibrous Scaffolds for Tissue Engineering
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DOI：
10.1021/am900048t
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发表时间：
2009-05-01
影响因子：
9.5
通讯作者：
Supaphol, Pitt
中科院分区：
文献类型：
--
作者：
Mattanavee, Waradda;Suwantong, Orawan;Supaphol, Pitt
为了使聚己内酯（PCL）更适用于组织工程，首先用1，6-己二胺对PCL进行改性，在其表面引入氨基，形成静电纺丝纤维支架。各种生物分子，即，胶原、壳聚糖和Gly-Arg-Gly-Asp-Ser（GRGDS）肽，然后用N，N ′-二琥珀酰亚胺基碳酸酯作为偶联剂固定在它们的表面上。动态水接触角的测量表明，支架表面变得更加亲水后的氨解处理和随后的生物分子的固定。这些PCL纤维支架用于组织/细胞培养的适当性在体外用三种不同的细胞系进行评价，例如，小鼠成纤维细胞（L929）、人表皮角质形成细胞（HEK 001）和小鼠颅骨衍生的前成骨细胞（MC 3 T3-E1）。纯的和改性的PCL纤维支架都没有释放对这些细胞有害的物质。在各种生物分子固定化的PCL纤维支架中，已经用1型胶原（一种含Arg-Gly-Asp的蛋白质）固定化的那些支架显示出最大的支持所有研究的细胞类型的附着和增殖的能力，其次是已经用GRGDS肽固定化的那些支架。
To make polycaprolactone (PCL) more suitable for tissue engineering, PCL in the form of electrospun fibrous scaffolds was first modified with 1,6-hexamethylenediamine to introduce amino groups on their surface. Various biomolecules, i.e., collagen, chitosan, and Gly-Arg-Gly-Asp-Ser (GRGDS) peptide, were then immobilized on their surface, with N,N'-disuccinimidylcarbonate being used as the coupling agent. Dynamic water contact angle measurement indicated that the scaffold surface became more hydrophilic after the aminolytic treatment and the subsequent immobilization of the biomolecules. The appropriateness of these PCL fibrous scaffolds for the tissue/cell culture was evaluated in vitro with three different cell lines, e.g., mouse fibroblasts (L929), human epidermal keratinocytes (HEK001), and mouse calvaria-derived preosteoblastic cells (MC3T3-E1). Both the neat and the modified PCL fibrous scaffolds released no substances in the levels that were harmful to these cells. Among the various biomolecule-immobilized PCL fibrous scaffolds, the ones that had been immobilized with type 1 collagen, a Arg-Gly-Asp-containing protein, showed the greatest ability to support both the attachment and the proliferation of all of the investigated cell types, followed by those that had been immobilized with GRGDS peptide.