Homotypic and heterotypic antibody responses to a 56-kilodalton protein of Orientia tsutsugamushi
Homotypic and heterotypic antibody responses to a 56-kilodalton protein of Orientia tsutsugamushi
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DOI：
10.1128/iai.67.11.6194-6197.1999
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发表时间：
1999-11-01
影响因子：
3.1
通讯作者：
Kim, IS
中科院分区：
文献类型：
--
作者：
Choi, MS;Seong, SY;Kim, IS
我们通过使用 Gilliam、Karp、Kato 和 Boryong 菌株免疫小鼠的血清，分析了对类型特异性抗原 (Tsa)（恙虫病东方体的 56 kDa 蛋白）的同型和异型抗体反应，我们生成了一系列 tsa 基因的缺失构建体，并将其表达为 MalE 融合蛋白。可变域 I (VD I) 对同型抗体表现出强烈的反应。 Boryong 和 Karp 的抗原结构域 II (AD II) 显示出彼此的交叉反应性。 VD III 对任何抗体都没有反应。加藤免疫小鼠的血清仅显示出对 AD III 的同型反应。另一方面，用Gilliam、Karp或Boryong免疫的小鼠血清显示出对该区域的同型和异型反应，VD IV在测试的片段中显示出最强的异型抗体反应。这些数据表明，VD I 在同型抗体反应中很重要，而 AD II、AD III 和 VD IV 在小鼠对 Tsa 的异型抗体反应中很重要。
We analyzed homotypic and heterotypic antibody responses to a type-specific antigen (Tsa), a 56-kDa protein of Orientia tsutsugamushi, by using sera from mice immunized with strains Gilliam, Karp, Kato, and Boryong, We generated a series of deletion constructs of the tsa gene and expressed them as MalE fusion proteins. Variable domain I (VD I) showed strong responses to homotypic antibodies. Antigenic domain II (AD II) from Boryong and Karp showed cross-reactivities to each other. VD III sheaved no responses to any of the antibodies. Sera from Kato-immunized mice showed only homotypic responses to AD III. On the other hand, sera of the mice immunized with Gilliam, Karp, or Boryong showed homotypic as well as heterotypic responses to this region, VD IV showed the strongest heterotypic antibody responses among the fragments tested. These data suggest that VD I is important in homotypic antibody responses and that AD II, AD III, and VD IV are important in heterotypic antibody responses of the mice to Tsa.