Regeneration of neuronal cell types in Schmidtea mediterranea: an immunohistochemical and expression study
Regeneration of neuronal cell types in Schmidtea mediterranea: an immunohistochemical and expression study
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DOI：
10.1387/ijdb.113428sf
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发表时间：
2012-01-01
影响因子：
0.7
通讯作者：
Cebria, Francesc
中科院分区：
文献类型：
--
作者：
Fraguas, Susanna;Barberan, Sara;Cebria, Francesc
淡水真涡虫的独特之处在于，它们能够在短短几天内从身体的几乎任何一小部分再生一个完整的中枢神经系统（CNS）。涡虫中枢神经系统包含一对位于两条腹神经索顶部的前头神经节，腹神经索沿沿着动物的长度延伸。涡虫中枢神经系统再生的研究一般使用泛神经标记，这只提供了一个一般的概述的过程。然而，一些报告已经开始描述这一过程所需的基因。在这项研究中，以获得更详细的描述，涡虫神经再生，我们监测的神经元群体的再生特异性标记的抗体对血清素，allatostatin，神经肽F，GYRFamide和FMRFamide。我们还通过原位杂交表征了多巴胺能和章鱼胺能细胞群体的再生。最后，我们表征了一组神经递质、神经肽和激素受体的表达模式，这些受体被认为在再生过程本身中发挥作用。总之，这些数据提供了一个更详细的描述细胞的事件发生在前部和后部的中枢神经系统再生的涡虫，并提供了基础，为未来的机制研究到再生过程中，在这个重要的模型系统。
Freshwater planarians are unique in their ability to regenerate a complete Central Nervous System (CNS) from almost any small piece of their body in just a few days. The planarian CNS contains a pair of anterior cephalic ganglia lying on top of two ventral nerve cords that extend along the length of the animal. Studies of planarian CNS regeneration have generally used pan-neural markers, which provide only a general overview of the process. Nevertheless, some reports have started to characterize the genes that are required for this process. In this study, to obtain a more detailed description of planarian neural regeneration, we monitored the regeneration of neuronal populations specifically labelled with antibodies against serotonin, allatostatin, neuropeptide F, GYRFamide and FMRFamide. We also characterized the regeneration of dopaminergic and octopaminergic cell populations by in situ hybridization. Finally, we characterized the expression pattern of a set of receptors for neurotransmitters, neuropeptides and hormones that are suggested to play a role in the regeneration process itself. Together, these data provide a more detailed description of the cellular events occurring during anterior and posterior CNS regeneration in planarians and provide the foundations for future mechanistic studies into the regeneration process in this important model system.