Regulation of the rat brain Na+-driven Cl-/HCO-3 exchanger involves protein kinase A and a multiprotein signaling complex
Regulation of the rat brain Na+-driven Cl-/HCO-3 exchanger involves protein kinase A and a multiprotein signaling complex
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DOI：
10.1016/j.febslet.2006.07.075
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发表时间：
2006-09-04
期刊：
影响因子：
3.5
通讯作者：
Giffard, Rona G.
中科院分区：
文献类型：
--
作者：
Lee, Yong-Sun;Ouyang, Yi-Bing;Giffard, Rona G.
Na+驱动的Cl-/HCO3-交换(NCBE)在调节细胞内pH(pH(I))中起着重要作用。我们以前从大鼠脑中发现了两种NCBE变体，其中带有PSD-95/DLG/ZO-1(PDZ)基序的变体(Rb2NCBE)与肌动蛋白细胞骨架共定位。PKA抑制Rb2NCBE活性，Forsklin和cAMP激动剂降低Rb2NCBE活性，提示PKA对NCBE有调节作用。肌动蛋白细丝的断裂也降低了rb2NCBE的活性。EBP50和FLAG-rb2NCBE从rb2NCBE转基因细胞中双向免疫共沉淀。结论：NCBE活性被PKA抑制，并依赖于质膜上多蛋白复合体中肌动蛋白细胞骨架的完整性。(C)2006年，由Elsevier B.V.代表欧洲生化学会联合会出版。
The Na+-driven Cl-/HCO3- exchanger (NCBE) plays an important role in the regulation of intracellular pH (pH(i)). We previously identified two variants of NCBE from rat brain of which the variant with a carboxyterminal PSD-95/Dlg/ZO-1 (PDZ) motif (rb2NCBE) colocalized with the actin cytoskeleton. Increased rb2NCBE activity by PKA inhibition and reduction by forskolin and cAMP agonist suggest PKA regulation of NCBE. Disruption of actin filaments also decreased rb2NCBE activity. EBP50 and FLAG-rb2NCBE were reciprocally co-immunoprecipitated from rb2NCBE transfected cells. It is concluded that NCBE activity is inhibited by PKA and depends on the integrity of the actin cytoskeleton within a multiprotein complex at the plasma membrane. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.