An optical method to quantify the density of ligands for cell adhesion receptors in three-dimensional matrices.
An optical method to quantify the density of ligands for cell adhesion receptors in three-dimensional matrices.
复制标题
一种定量三维基质中细胞粘附受体配体密度的光学方法。
DOI：
10.1098/rsif.2010.0321.focus
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Yannas,IoannisV
中科院分区：
文献类型：
--
作者：
Tzeranis,DimitriosS;Roy,Amit;So,PeterTC;Yannas,IoannisV
包围细胞的三维基质是细胞表型的重要不溶性调节剂。这种不溶性表面的实例是细胞外基质（ECM）、ECM类似物和合成聚合物生物材料。细胞-基质相互作用由细胞粘附受体介导，细胞粘附受体结合基质表面上的化学实体（粘附配体）。目前还没有确定的方法来获得由特异性细胞粘附受体识别的粘附配体密度的定量估计。本文提出了一种新的基于光学的方法来测量三维矩阵上的粘附受体的配体。该研究还提供了胶原基支架表面整合素α1β 1和α2β 1粘附配体密度的初步定量结果，类似于临床上用于诱导损伤皮肤和外周神经再生的生物材料。这两种主要胶原结合受体的配体表面密度的初步估计值为：α1β 1配体为5775 ± 2064 μm− 2，α2β1配体为17 084 ± 5353 μm− 2。所提出的方法可用于量化具有生物活性的不溶性表面的表面化学，例如天然组织ECM和生物材料，因此可用于细胞生物学、生物材料科学和再生医学研究，用于定量描述基质及其对细胞的影响。
The three-dimensional matrix that surrounds cells is an important insoluble regulator of cell phenotypes. Examples of such insoluble surfaces are the extracellular matrix (ECM), ECM analogues and synthetic polymeric biomaterials. Cell–matrix interactions are mediated by cell adhesion receptors that bind to chemical entities (adhesion ligands) on the surface of the matrix. There are currently no established methods to obtain quantitative estimates of the density of adhesion ligands recognized by specific cell adhesion receptors. This article presents a new optical-based methodology for measuring ligands of adhesion receptors on three-dimensional matrices. The study also provides preliminary quantitative results for the density of adhesion ligands of integrinsα1β1andα2β1on the surface of collagen-based scaffolds, similar to biomaterials that are used clinically to induce regeneration in injured skin and peripheral nerves. Preliminary estimates of the surface density of the ligands of these two major collagen-binding receptors are 5775 ± 2064 ligands µm−2for ligands ofα1β1and 17 084 ± 5353 ligands µm−2for ligands ofα2β1. The proposed methodology can be used to quantify the surface chemistry of insoluble surfaces that possess biological activity, such as native tissue ECM and biomaterials, and therefore can be used in cell biology, biomaterials science and regenerative medical studies for quantitative description of a matrix and its effects on cells.