Increased Apoptosis in Chorionic Trophoblasts of Human Fetal Membranes with Labor at Term.
Increased Apoptosis in Chorionic Trophoblasts of Human Fetal Membranes with Labor at Term.
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DOI：
10.4236/ijcm.2012.32027
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hankins GD
中科院分区：
文献类型：
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作者：
Harirah HM;Borahay MA;Zaman W;Ahmed MS;Hankins GD
目的探讨人胎膜细胞凋亡与足月分娩的关系。从择期剖宫产（n = 8）和自然阴道分娩（n = 8）中收集足月人胎膜。采用末端脱氧核苷酸转移酶脱氧-UTP-缺口末端标记（TUNEL）免疫组化法和蛋白质印迹法测定胎膜各层细胞凋亡的程度。对于TUNEL试验，使用福尔马林固定膜的5 μm切片，并在5个独立的显微镜视野中测定凋亡指数（凋亡细胞核数/总细胞核×100）。对于蛋白质印迹法，将从羊水和绒毛膜蜕膜层分离的蛋白质针对促细胞凋亡活性半胱天冬酶-3和抗细胞凋亡蛋白酶和Bcl-2进行印迹。数据表示为平均值± SD，使用Student t检验进行统计分析。在足月分娩的患者中，选择性剖宫产或阴道分娩的患者在母亲年龄、胎龄、妊娠次数、产次、种族和吸烟方面没有统计学差异。阴道分娩后获得的绒毛膜滋养层细胞的凋亡指数高于择期剖宫产获得的绒毛膜滋养层细胞的凋亡指数（分别为11.57 ± 4.98%和4.05 ± 2.4%，p = 0.012）。阴道分娩后的绒毛膜蜕膜层有较高的表达的促凋亡活性caspase-3和表达的抗凋亡BcL-2比那些从选择性剖宫产。足月分娩与人胎膜绒毛膜滋养层细胞凋亡增加有关。胎膜细胞凋亡与分娩的因果关系值得进一步研究。
To determine the association of apoptosis in the layers of human fetal membranes with labor at term. Human fetal membranes were collected from elective cesarean sections (n = 8) and spontaneous vaginal deliveries (n = 8) at term. The extent of apoptosis within the layers of fetal membranes was determined using terminal deoxynucleotidyl transferase deoxy-UTP-nick end labeling (TUNEL) immunohistochemical assay and western blots. For TUNEL assays, 5-μm sections of formalin fixed membranes were used and the apoptotic index (number of apoptotic nuclei per total nuclei ×100) was determined in 5 independent microscopic fields. For Western blotting, proteins isolated from the amnion and choriodecidua layers were blotted against pro-apoptotic active caspase-3 and anti-apoptotic and Bcl-2. Data were expressed as the means ± SD and Student’s t-test was used for statistical analysis. There was no statistical difference in maternal age, gestational age, gravidity, parity, race, and smoking between patients who delivered at term via either elective cesarean or vaginally. Apoptotic index in chorionic trophoblasts of membranes obtained after vaginal delivery was higher than those obtained from elective cesarean (11.57 ± 4.98 % and 4.05 ± 2.4 % respectively, p = 0.012). The choriodecidua layers after vaginal deliveries had higher expression of the pro-apoptotic active caspase-3 and less expression of the anti-apoptotic BcL-2 than those obtained from elective cesarean sections. Labor at term is associated with increased apoptosis in chorionic trophoblast cells of human fetal membranes. The cause-effect relation between apoptosis in fetal membranes and labor warrants further investigations.