Total Chemical Synthesis of All SUMO-2/3 Dimer Combinations
Total Chemical Synthesis of All SUMO-2/3 Dimer Combinations
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DOI：
10.1021/acs.bioconjchem.9b00661
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发表时间：
2019-11-01
影响因子：
4.7
通讯作者：
Melnyk, Oleg
中科院分区：
文献类型：
--
作者：
Bouchenna, Jennifer;Senechal, Magalie;Melnyk, Oleg
蛋白质化学合成的一个标志是它能够获得生命系统难以产生的蛋白质。这通常是具有翻译后修饰的蛋白质如泛素或泛素样修饰剂的情况。已经开发了通过半合成或全合成获得聚泛素缀合物的各种方法。相比之下，小泛素样修饰物（SUMO）缀合物的制备，更具体地，聚SUMO支架的制备，开发得少得多。我们在下文中描述了用于获得所有SUMO-2/3二聚体组合的合成策略。
One hallmark of protein chemical synthesis is its capacity to access proteins that living systems can hardly produce. This is typically the case for proteins harboring post-translational modifications such as ubiquitin or ubiquitin-like modifiers. Various methods have been developed for accessing polyubiquitin conjugates by semi- or total synthesis. Comparatively, the preparation of small-ubiquitin-like modifier (SUMO) conjugates, and more particularly of polySUMO scaffolds, is much less developed. We describe hereinafter a synthetic strategy for accessing all SUMO-2/3 dimer combinations.