A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo.
A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo.
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DOI：
10.1182/blood-2014-02-559468
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发表时间：
2014-10
期刊：
影响因子：
20.3
通讯作者：
K. Potts;T. Sargeant;J. Markham;Wei Shi;C. Biben;E. Josefsson;L. Whitehead;K. Rogers;Anna Liakhovitskaia;G. Smyth;B. Kile;A. Medvinsky;W. Alexander;D. Hilton;S. Taoudi
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文献类型：
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作者：
K. Potts;T. Sargeant;J. Markham;Wei Shi;C. Biben;E. Josefsson;L. Whitehead;K. Rogers;Anna Liakhovitskaia;G. Smyth;B. Kile;A. Medvinsky;W. Alexander;D. Hilton;S. Taoudi
在这项研究中，我们验证了胚胎卵黄囊(YS)中具有巨核细胞潜能的造血祖细胞/集落形成细胞在体内分化为第一批血小板形成细胞的假设。我们证明从胚胎8.5天起，所有的巨核细胞(MK)集落形成细胞都属于传统的造血祖细胞(HPC)室。虽然这些细胞确实能够产生多倍体的巨噬细胞，但它们不是第一批血小板形成细胞的来源。我们表明，原血小板的形成首先发生在一个独特的和以前未被识别的二倍体血小板形成细胞谱系中，这些细胞在YS内平行于HPC发育，但可以在E8.5 RUNX1缺失的胚胎中指定，尽管没有祖细胞谱系。
In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.