Prevalence of HLA-B27 in Moroccan healthy subjects and patients with ankylosing spondylitis and mapping construction of several factors influencing AS diagnosis by using multiple correspondence analysis
Prevalence of HLA-B27 in Moroccan healthy subjects and patients with ankylosing spondylitis and mapping construction of several factors influencing AS diagnosis by using multiple correspondence analysis
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DOI：
10.1007/s00296-015-3342-x
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发表时间：
2015-11-01
影响因子：
4
通讯作者：
Bakri, Youssef
中科院分区：
文献类型：
--
作者：
Akassou, Amal;Yacoubi, Hanae;Bakri, Youssef
本研究的目的是确定摩洛哥健康对照组和强直性脊柱炎（AS）患者中人类白细胞抗原HLA-B27的患病率，并分析摩洛哥患者HLA-B27与AS之间的相关性。通过评估128名健康受试者和53名根据ESSG和AMOR标准诊断为AS的患者中HLA-B27阳性样本的数量来确定HLA-B27的患病率。HLA-B27通过使用序列特异性引物的聚合酶链反应测定。我们的数据（HLA-B27，年龄，性别和家族史）的AS和健康对照组进行了多变量分析（MCA）。AS患者HLA-B27的频率（45.3%）显著高于健康对照组（4.7%）[p < 0.0001，OR 16.8，CI 95%（5.83-51.03）]。HLA-B27阳性率男性高于女性（P < 0.05）。100%的AS患者报告有AS家族史，而仅20%的健康对照报告有AS家族史。MCA的图形解释显示HLA-B27的存在与AS之间存在显著相关。这项研究加强了HLA-B27和AS之间的联系，并代表了一个非常有价值的信息诊断工具，特别是在有AS家族史的男性患者。
The aim of the present study was to determine the prevalence of human leukocyte antigen HLA-B27 in Moroccan healthy controls and in patients with ankylosing spondylitis (AS), and to analyze the correlation between HLA-B27 and AS in Moroccan patients. The prevalence of HLA-B27 was determined by evaluating the number of HLA-B27-positive samples in 128 healthy subjects and in 53 patients diagnosed with AS according to the ESSG and AMOR criteria. HLA-B27 was determined by the polymerase chain reaction using sequence-specific primers. Multivariate analysis of our data (HLA-B27, age, sex, and family history) for AS and healthy controls was performed by multiple correspondence analysis (MCA). The frequency of HLA-B27 was significantly greater in AS patients (45.3 %) than in healthy controls (4.7 %) [p < 0.0001, OR 16.8, and CI 95 % (5.83-51.03)]. In addition, HLA-B27 was more common in male patients than in female ones (p < 0.05). 100 % of the AS patients reported a family history of AS, whereas only 20 % of the healthy controls reported a family history of AS. The graphical interpretation of MCA showed a significant relation between the presence of HLA-B27 and AS. This study strengthens the link between HLA-B27 and AS and represents a very valuable informative diagnostic tool, especially in regard to male patients who have a family history of AS.