Challenges and new opportunities for detecting endogenous opioid peptides in reward.
Challenges and new opportunities for detecting endogenous opioid peptides in reward.
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DOI：
10.1016/j.addicn.2022.100016
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发表时间：
2022-06-01
期刊：
影响因子：
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通讯作者：
Al-Hasani, Ream
中科院分区：
文献类型：
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作者：
Conway, Sineadh M;Mikati, Marwa O;Al-Hasani, Ream
内源性阿片肽系统由脑啡肽、内啡肽、强啡肽和伤害感受素组成，是一个高度复杂的神经生物学系统。阿片肽衍生自四种前体分子，并经历若干加工事件，产生超过20种独特的阿片肽。这种多样性与低体内浓度和复杂的加工和释放动力学一起挑战了对每种肽的独特功能的研究。尽管随后在体内检测和定量阿片肽方面存在挑战，但研究人员已经开创了几种技术来直接或间接测定阿片肽在行为操纵过程中的作用。在这篇综述中，我们描述了用于研究内源性阿片肽在食物和药物奖励中的作用的传统技术的局限性，并将重点放在丰富的新技术来测量内源性阿片肽在奖励处理。
The endogenous opioid peptide system, comprised of enkephalins, endorphins, dynorphins, and nociceptin, is a highly complex neurobiological system. Opioid peptides are derived from four precursor molecules and undergo several processing events yielding over 20 unique opioid peptides. This diversity together with low in vivo concentration and complex processing and release dynamics has challenged research into each peptide's unique function. Despite the subsequent challenges in detecting and quantifying opioid peptides in vivo, researchers have pioneered several techniques to directly or indirectly assay the roles of opioid peptides during behavioral manipulations. In this review, we describe the limitations of the traditional techniques used to study the role of endogenous opioid peptides in food and drug reward and bring focus to the wealth of new techniques to measure endogenous opioid peptides in reward processing.