Suppression of Bedbug's Reproduction by RNA Interference of Vitellogenin.
Suppression of Bedbug's Reproduction by RNA Interference of Vitellogenin.
复制标题
DOI：
10.1371/journal.pone.0153984
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Fukatsu T
中科院分区：
文献类型：
--
作者：
Moriyama M;Hosokawa T;Tanahashi M;Nikoh N;Fukatsu T
最近臭虫Cimex lectularius的死灰复燃是一个全球性的公共卫生问题。随着世界范围内耐杀虫剂臭虫种群的增加，人们期待着探索新的方法来控制和管理臭虫。在这种背景下，RNA干扰(RNAi)的基因沉默因其在害虫控制和管理中的潜在应用而被认为是潜在的，因为RNAi使目标基因的特异性抑制，从而扰乱了目标性状的灵活选择。在这项研究中，为了开发一种针对臭虫繁殖的控制策略，我们研究了RNAi介导的卵黄蛋白原(VG)的基因沉默，卵黄蛋白原是卵子发生所必需的主要卵黄蛋白前体。从臭虫的转录本中，我们发现了一个典型的VG基因和一个截短的VG基因，分别命名为ClVg和ClVg-like。ClVg基因主要在雌性成虫脂肪体中高表达，是类ClVg基因表达水平的100多倍，表明ClVg基因是臭虫主要的功能性Vg基因。RNAi介导的ClVg基因在成年雌性中的表达抑制导致卵子产量急剧减少，卵巢萎缩，以及由于肥大的脂肪体而导致的腹部膨胀。预计这些表型后果不仅会直接抑制臭虫的繁殖，还会通过行为改变间接恶化其取食和生存。这些结果表明，ClVg基因有可能成为基于RNAi的床虱种群管理的潜在靶点。
Recent resurgence of the bedbug Cimex lectularius is a global problem on the public health. On account of the worldwide rise of insecticide-resistant bedbug populations, exploration of new approaches to the bedbug control and management is anticipated. In this context, gene silencing by RNA interference (RNAi) has been considered for its potential application to pest control and management, because RNAi enables specific suppression of target genes and thus flexible selection of target traits to be disrupted. In this study, in an attempt to develop a control strategy targeting reproduction of the bedbug, we investigated RNAi-mediated gene silencing of vitellogenin (Vg), a major yolk protein precursor essential for oogenesis. From the bedbug transcriptomes, we identified a typical Vg gene and a truncated Vg gene, which were designated as ClVg and ClVg-like, respectively. ClVg gene was highly expressed mainly in the fat body of adult females, which was more than 100 times higher than the expression level of ClVg-like gene, indicating that ClVg gene is the primary functional Vg gene in the bedbug. RNAi-mediated suppression of ClVg gene expression in adult females resulted in drastically reduced egg production, atrophied ovaries, and inflated abdomen due to hypertrophied fat bodies. These phenotypic consequences are expected not only to suppress the bedbug reproduction directly but also to deteriorate its feeding and survival indirectly via behavioral modifications. These results suggest the potential of ClVg gene as a promising target for RNAi-based population management of the bedbug.