Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan:: isolation and characterization of hydrocarbon-degrading bacteria
Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan:: isolation and characterization of hydrocarbon-degrading bacteria
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DOI：
10.1016/j.envint.2004.02.007
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发表时间：
2004-09-01
影响因子：
11.8
通讯作者：
Kogure, K
中科院分区：
文献类型：
--
作者：
Chaerun, SK;Tazaki, K;Kogure, K
在1997年日本海纳霍德卡漏油事件发生5年后，经过5年的生物修复过程，从三个沿海地区（即福井县的片野海岸、石川县的大泽和武竹海岸）和纳霍德卡俄罗斯油轮上分离出了7种能够利用纳霍德卡俄罗斯油轮泄漏的重油的细菌菌株。所有分离的菌株都能有效地利用长链烷烃，但不能有效地利用芳烃，并且所有菌株都能在重油上生长良好。16S rDNA测序结果显示，大部分菌株属于铜绿假单胞菌。与1997年（生物修复开始时）和2001年（生物修复5年后）相比，在5年的生物修复过程中，烃类降解菌的形态和大小没有明显变化。扫描电镜和透射电镜观察显示，存在大量烃类降解菌。仍然存在于由多种形态的细菌组成的部位，如球菌（链球菌和葡萄球菌）和芽孢杆菌（链球菌）。针对生物修复工艺在实验室规模上的应用，在好氧条件下（含海水、沙滩砂和重油）建立了两种不同处理（建筑内部和建筑外部）的实验室微观实验，对重油进行生物修复，探讨烃降解菌的作用意义。在他们身上。两种处理没有显著的细菌活性分化，建筑物外烃类降解菌对重油的去除量略大于建筑物内。两种处理的pH、Eh、EC和溶解氧（DO）值表明，生物修复过程发生在好氧条件下（DO: 1 ~ 6 mg/l, Eh: 12 ~ 300 mV）和中碱性条件下（pH 6.4 ~ 8）， NaCl浓度为3 ~ 15% （ECs为45 ~ 200 mS/cm）。(C) 2004 Elsevier Ltd.版权所有。
Five years after the 1997 Nakhodka oil spill in the Sea of Japan, seven bacterial strains capable of utilizing the heavy oil spilled from the Nakhodka Russian oil tanker were isolated from three coastal areas (namely Katano Seashore of Fukui Prefecture, Osawa and Atake seashores of Ishikawa Prefecture) and the Nakhodka Russian oil tanker after a 5-year bioremediation process. All bacterial strains isolated could utilize long-chain-length alkanes efficiently, but not aromatic, and all of them were able to grow well on heavy oil. Using 16S rDNA sequencing, most of the strains were affiliated to Pseudomonas aeruginosa. Comparing between the year 1997 (at the beginning of bioremediation process) and the year 2001 (after 5 years of bioremediation), there was no significant change in morphology and size of hydrocarbon-degrading bacteria during the 5-year bioremediation. Scanning and transmission electron microscopic observations revealed that a large number of hydrocarbondegrading bacteria. still existed in the sites consisting of a variety of morphological forms of bacteria, such as coccus (Streptococcus and Staphylococcus) and bacillus (Streptobacillus). On the application of bioremediation processes on the laboratory-scale, laboratory microcosm experiments (containing seawater, beach sand, and heavy oil) under aerobic condition by two different treatments (i.e., placed the inside building and the outside building) were established for bioremediation of heavy oil to investigate the significance of the role of hydrocarbondegrading bacteria. on them. There was no significant bacterial activity differentiation in the two treatments, and removal of heavy oil by hydrocarbon-degrading bacteria in the outside building was slightly greater than that in the inside building. The values of pH, Eh, EC, and dissolved oxygen (DO) in two treatments indicated that the bioremediation process took place under aerobic conditions (DO: 1-6 mg/l; Eh: 12-300 mV) and neutral-alkaline conditions (pH 6.4-8) with NaCl concentrations of 3-15% (ECs of 45-200 mS/cm). (C) 2004 Elsevier Ltd. All rights reserved.