The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1
The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1
复制标题
前列腺素E1保护肝移植物抗再灌注损伤的机制
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
K. Sugimachi
中科院分区：
文献类型：
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作者：
H. Itasaka;T. Suehiro;S. Wakiyama;K. Yanaga;M. Shimada;K. Sugimachi
本研究的目的是评价前列腺素E1（PGE 1）对保护肝内皮细胞损伤和提高移植物活力的影响，冷保存和再灌注后，使用离体灌注大鼠肝脏（IPRL）模型。根据冷保存时间和PGE 1输注情况，将移植物分为3组：保存4 h组（1组，n=9）、保存6 h组（2组，n=9）和保存6 h后输注PGE 1组（3组，n=9）。冷藏后，将移植物置于再循环IPRL系统上，然后在37°C下用含透明质酸（HA）的充氧Krebs-Henseleit缓冲液再灌注120分钟。为检测肝窦内皮细胞和肝细胞的功能，对灌流液中HA、肿瘤坏死因子-α（TNFα）、血栓素B2（TXB 2）、酸性磷酸酶和常规参数进行了系列检测。灌注后，进行台盼蓝排除试验，以评估是否存在任何显微镜下窦状隙衬里细胞损伤。第3组胆汁排出量和HA清除率显著高于第2组，而灌流液中谷草转氨酶、谷草转氨酶、TNFα、TXB 2和酸性磷酸酶显著低于第2组。在组织学上，也证实了比第2组更少的内皮细胞损伤和肝细胞损伤。因此，这些结果表明，通过给予PGE 1来改善肝移植物存活率主要是由于窦状内皮细胞保护。
The purpose of this study was to evaluate the effect of prostaglandin E1 (PGE1) on protecting against hepatic endothelial cell damage and increasing graft viability after cold preservation and reperfusion, using an isolated perfused rat liver (IPRL) model. The grafts were divided into three groups, according to the cold preservation time and PGE1 administration, namely: 4h preservation (group 1,n=9), 6h preservation (group 2,n=9), and 6h preservation followed by PGE1 infusion (group 3,n=9). After cold storage, the grafts were put on the recirculating IPRL system, then reperfused for 120 min at 37°C with oxygenated Krebs-Henseleit buffer containing hyaluronic acid (HA). To examine the function of the sinusoidal endothelial cells and hepatocytes, serial measurements of HA, tumor necrosis factor-α (TNFα), thromboxane B2 (TXB2), acid phosphatase, and conventional parameters in the perfusate were made. After perfusion, the trypan blue exclusion test was performed to assess the presence of any microscopic sinusoidal lining cell damage. In group 3, the bile output and HA clearance were significantly greater, while glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, TNFα, TXB2, and acid phosphatase in the perfusate were significantly lower than in group 2. Histologically, less endothelial cell damage and hepatocyte damage than in group 2 was also confirmed. These results therefore suggest that the improvement of hepatic graft viability by PGE1 administration is mainly due to sinusoidal endothelial cell protection.
有证据表明，大鼠肝移植中移植物存活与实质细胞活力无关。
影响因子：
6.2
作者：
Marzi,I;Zhong,Z;Lemasters,JJ;Thurman,RG
通讯作者：
Thurman,RG
台盼蓝摄取作为研究肝小叶门静脉周围和中央周围区域肝毒性的新方法：灌注肝脏中烯丙醇的研究。
DOI：
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发表时间：
1984
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
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作者：
Belinsky,SA;Popp,JA;Kauffman,FC;Thurman,RG
通讯作者：
Thurman,RG