Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from Portugal
Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from Portugal
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DOI：
10.1016/j.vetpar.2012.12.060
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发表时间：
2013-09-01
影响因子：
2.6
通讯作者：
Baneth, G.
中科院分区：
文献类型：
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作者：
Cardoso, L.;Cortes, H. C. E.;Baneth, G.
采用分子方法对来自葡萄牙北部、中部和南部的91只赤狐（Vulpes Vulpes）的血液和骨髓样本中的螺质（螺质目）感染情况进行了评估。对巴贝斯虫18S rRNA基因进行PCR检测和测序，结果显示，有63只狐狸的piroplasm巴贝斯虫阳性（69.2%，95%可信区间[CI]: 58.7 ~ 78.5%）， 1只狐狸的犬巴贝斯虫阳性（1.1%,95% CI: 0.0 ~ 6.0%）。43份血样（83.7%）与43份配对骨髓样本（20.9%）相比，有显著性差异（p < 0.001）。感染患病率在性别（p = 0.219）和年龄组（p = 2岁）之间差异无统计学意义（p = 1.0）。这是首次在葡萄牙狐狸中发现细小螺旋体样螺旋体的报告，也是世界上首次在红狐中检测到犬螺旋体的PCR和基因分型。基于原生动物的高流行率，提示红狐种群中存在微螺旋体样螺质的自然循环。红狐可能是微螺旋体样螺旋体的宿主，也是犬类的传染源。(c) 2013 Elsevier B.V.版权所有
The prevalence of piroplasm (order Piroplasmida) infection was assessed in blood and bone marrow samples from 91 red foxes (Vulpes vulpes) from northern, central and southern Portugal by means of molecular methods. PCR for the 18S rRNA gene of Babesia spp. followed by sequencing revealed 63 foxes positive for the Babesia piroplasm (syn. Theileria annae) (69.2%; 95% confidence interval [CI]: 58.7-78.5%) and one fox positive for Babesia canis (1.1%; 95% CI: 0.0-6.0%). Positivity to the B. microti-like piroplasm or B. cools in 43 blood samples (83.7%) was significantly higher (p < 0.001) than in 43 paired bone marrow samples (20.9%). There were no statistically significant differences in the prevalence of infection between genders (p = 0.219) or age groups (= 2 years) (p = 1.0). This is the first report of the B. microti-like piroplasm in foxes from Portugal as well as the first report on detection by PCR and genotyping of B. canis in a red fox worldwide. A natural cycle of the B. microti-like piroplasm is suggested in red fox populations based on the high prevalence of the protozoan. Red foxes might be a reservoir of the B. microti-like piroplasm and a source of infection to dogs. (c) 2013 Elsevier B.V. All rights reserved.