Comparative studies of intracellular transport of secretory proteins.
Comparative studies of intracellular transport of secretory proteins.
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分泌蛋白细胞内转运的比较研究。
DOI：
10.1083/jcb.79.3.694
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发表时间：
1978-12
影响因子：
7.8
通讯作者：
Detraz, M
中科院分区：
文献类型：
--
作者：
Tartakoff, A;Vassalli, P;Detraz, M
被认为是免费的短期控制的细胞类型的蛋白质细胞内运输和分泌的生理学已与胰腺腺泡细胞进行了比较，使用脉冲追踪协议，以遵循生物合成标记的分泌产物。先前获得的数据（Tartakoff，A.M.，和P. Vassalli。J. Exp. 146：1332-1345）已经表明，在乙二醇双（β-氨基乙基醚）-N，N，N ′，N ′-四乙酸（EGTA）存在且不存在钙的情况下，通过呼吸抑制剂、通过由羧基离子载体莫能菌素实现的部分Na/K平衡、以及通过由羧基离子载体A 23187实现的钙撤回来抑制浆细胞免疫球蛋白（IG）分泌。我们在这里报告，无论是抑制呼吸和治疗莫能菌素缓慢分泌的成纤维细胞，巨噬细胞和缓慢的细胞内运输（虽然不是放电本身）的外分泌胰腺细胞。尝试脱钙对成纤维细胞有抑制作用，但对巨噬细胞无抑制作用。细胞外钙的消除或添加50 mM KCl对成纤维细胞或巨噬细胞的分泌速率没有重大影响。所有细胞类型的电子显微镜检查表明，莫能菌素引起高尔基体的快速和令人印象深刻的扩张。结合胰腺的细胞分级和放射自显影研究表明，莫能菌素的效果施加在分泌蛋白质从高尔基体的出口点。细胞内转运的其他步骤以正常速率进行。这些观察结果表明，在高尔基体水平的细胞质Na/K平衡的共同作用，并导致细胞内运输的模型，其中分泌产物强制性地通过高尔基体元件（cisterlavirus？）对莫能菌素敏感的细胞因此，细胞内运输遵循一个类似的过程，在调节和非调节分泌细胞的水平上的远端高尔基体元件。
The physiology of protein intracellular transport and secretion by cell types thought to be free from short-term control has been compared with that of the pancreatic acinar cell, using pulse-chase protocols to follow biosynthetically-labeled secretory products. Data previously obtained (Tartakoff, A.M., and P. Vassalli. J. Exp. Med. 146:1332-1345) has shown that plasma-cell immunoglobulin (Ig) secretion is inhibited by respiratory inhibitors, by partial Na/K equilibration effected by the carboxylic ionophore monensin, and by calcium withdrawal effected by the carboxylic ionophore A 23187 in the presence of ethylene glycol bis (beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and absence of calcium. We report here that both inhibition of respiration and treatment with monensin slow secretion by fibroblasts, and also macrophages and slow intracellular transport (though not discharge per se) by the exocrine pancreatic cells. Attempted calcium withdrawal is inhibitory for fibroblasts but not for macrophages. The elimination of extracellular calcium or addition of 50 mM KCl has no major effect on secretory rate of either fibroblasts or macrophages. Electron microscopic examination of all cell types shows that monensin causes a rapid and impressive dilation of Golgi elements. Combined cell fractionation and autoradiographic studies of the pancreas show that the effect of monensin is exerted at the point of the exit of secretory protein from the Golgi apparatus. Other steps in intracellular transport proceed at normal rates. These observations suggest a common effect of the cytoplasmic Na/K balance at the Golgi level and lead to a model of intracellular transport in which secretory product obligatorily passes through Golgi elements (cisternae?) that are sensitive to monensin. Thus, intracellular transport follows a similar course in both regulated and nonregulated secretory cells up to the level of distal Golgi elements.