Amelotin Promotes Mineralization and Adhesion in Collagen-Based Systems
Amelotin Promotes Mineralization and Adhesion in Collagen-Based Systems
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DOI：
10.1007/s12195-022-00722-2
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发表时间：
2022-03
影响因子：
2.8
通讯作者：
Y. Ikeda;J. Holcroft;Eri Ikeda;B. Ganss
中科院分区：
文献类型：
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作者：
Y. Ikeda;J. Holcroft;Eri Ikeda;B. Ganss
前言牙周炎是以牙槽骨等牙齿支持组织的破坏为特征的疾病。屏障膜在牙科中用于组织再生治疗。然而，常规膜具有与膜稳定性和骨矿化的直接诱导相关的问题。釉蛋白（Amelotin，AMTN）是一种釉基质蛋白，调节羟基磷灰石晶体的成核和生长。为了应用AMTN膜在临床实践中，我们研究了矿化和粘附作用的重组人（rh）AMTNin vitrousing胶原为基础的system.MethodsCollagen水凝胶纳入rhAMTN（AMTN凝胶）和rhAMTN-coated牙本质切片制备。然后将AMTN凝胶施加在商业膜（AMTN膜）上。将样品在矿化缓冲液中孵育长达24小时，并观察结构。测量牙本质与AMTN膜之间的最大粘结强度。使用酶联免疫吸附试验，rhAMTN的释放动力学从membranes.ResultsThe AMTN凝胶导致羟基磷灰石沉积物的形成上和内的胶原基质。此外，用rhAMTN涂覆牙本质表面促进表面上的矿物沉积物的沉淀。有趣的是，在AMTN膜中观察到位点特异性矿化。只有1%的rhAMTN从膜中释放出来。因此，AMTN膜粘附到牙本质表面的拉伸强度比检测到的rhAMTN-免费的屏障membrane.ConclusionsRhAMTN可以加速矿化和粘附在胶原蛋白为基础的系统的两倍以上。此外，AMTN膜可以为钙化组织再生材料的优化设计提供信息。
IntroductionPeriodontitis is characterized by the destruction of tooth-supporting tissues including the alveolar bone. Barrier membranes are used in dentistry for tissue regenerative therapy. Nevertheless, conventional membranes have issues related to membrane stability and direct induction of bone mineralization. Amelotin (AMTN), an enamel matrix protein, regulates hydroxyapatite crystal nucleation and growth. To apply an AMTN membrane in clinical practice, we investigated the mineralizing and adhesive effects of recombinant human (rh) AMTNin vitrousing a collagen-based system.MethodsCollagen hydrogel incorporated with rhAMTN (AMTN gel) and rhAMTN-coated dentin slices were prepared. AMTN gel was then applied on a commercial membrane (AMTN membrane). Samples were incubated for up to 24 h in mineralization buffer, and the structures were observed. The peak adhesive tensile strength between the dentin and AMTN membrane was measured. Using an enzyme-linked immunosorbent assay, the release kinetics of rhAMTN from the membrane were investigated.ResultsThe AMTN gel resulted in the formation of hydroxyapatite deposits both onto and within the collagen matrix. Furthermore, coating the dentin surface with rhAMTN promoted the precipitation of mineral deposits on the surface. Interestingly, site-specific mineralization was observed in the AMTN membrane. Only 1% of rhAMTN was released from the membrane. Hence, the AMTN membrane adhered to the dentin surface with more than twofold greater tensile strength than that detected for a rhAMTN-free barrier membrane.ConclusionsRhAMTN can accelerate mineralization and adhesion in collagen-based systems. Furthermore, the AMTN membrane could inform the optimal design of calcified tissue regenerative materials.