Complete genome sequence of a novel mitovirus from the ectomycorrhizal fungus Geopora sumneriana
Complete genome sequence of a novel mitovirus from the ectomycorrhizal fungus Geopora sumneriana
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DOI：
10.1007/s00705-019-04367-x
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发表时间：
2019-11-01
影响因子：
2.7
通讯作者：
Akata, Ilgaz
中科院分区：
文献类型：
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作者：
Sahin, Ergin;Akata, Ilgaz
从外生菌根真菌Geopora sumneriana (Cooke) M. Torre中提取双链RNA (dsRNA)片段，并测定其全长cDNA序列，包含3146个核苷酸。序列分析表明，当应用霉菌线粒体遗传密码时，该 dsRNA 片段的正链上存在一个大的开放阅读框 (ORF)。 ORF编码一种推定的RNA依赖的RNA聚合酶(RdRp)，其与漏斗块菌线粒体病毒具有最高程度的相似性，具有37.52%的同一性。该 dsRNA 片段代表了一种新型线粒体病毒的基因组复制中间体，该病毒暂时被命名为“Geopora sumneriana 线粒体病毒 1”(GsMV1)。系统发育分析进一步表明，GsMV1 是纳纳病毒科 (Narnaviridae) 的成员。这是第一项关于外生菌根真菌 G. sumneriana 线粒体病毒基因组序列的研究。
A double-stranded RNA (dsRNA) segment was extracted from the ectomycorrhizal fungus Geopora sumneriana (Cooke) M. Torre, and its full-length cDNA sequence, comprising 3146 nucleotides, was determined. Sequence analysis revealed the presence of a large open reading frame (ORF) on the positive strand of this dsRNA segment when the mold mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase (RdRp), which shares the highest degree of similarity with Tuber excavatum mitovirus, with 37.52% identity. This dsRNA segment represents the genome replication intermediate of a novel mitovirus that was tentatively designated as "Geopora sumneriana mitovirus 1" (GsMV1). Phylogenetic analysis further suggested that GsMV1 is a member of the family Narnaviridae. This is the first study reporting on a mitovirus genome sequence in the ectomycorrhizal fungus G. sumneriana.