Physical mapping of male fertility and meiotic drive quantitative trait loci in the mouse t complex using chromosome deficiencies.
Physical mapping of male fertility and meiotic drive quantitative trait loci in the mouse t complex using chromosome deficiencies.
复制标题
利用染色体缺陷对小鼠 t 复合体中雄性生育力和减数分裂驱动数量性状基因座进行物理绘图。
DOI：
10.1093/genetics/155.2.803
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Schimenti,JC
中科院分区：
文献类型：
--
作者：
Planchart,A;You,Y;Schimenti,JC
该复合体跨越小鼠17号染色体近端区域的20 cM。一种变异形式，thaplotype（t），在野生小鼠种群中以显着的频率存在，其特征是存在倒位，抑制与野生型（+）染色体的重组。传递率畸变和不育只影响雄性.据推测，这些现象是由trans-acting扭曲/不育因子与反应位点（Tcrt）相互作用引起的，并且扭曲因子和不育因子是相同的，因为扭曲因子的纯合性导致雄性不育。一个因素，Tcd 1，以前被证明是无定形使用染色体缺失。为了克服重组抑制的局限性，我们使用了一系列的缺失内的复合物transotchromosomes来表征Tcd 1区域。我们发现Tcd 1的畸变活性不同于一个最初被称为dts 1的连锁不育因子。YAC相对于缺失断裂点定位于D17 Aus 9和Tctex 1两侧的1.1 Mb区间。我们提出了存在多个表现畸变活性的邻近复合物区域的证据。这些研究证明了染色体缺失在复杂性状分析中的实用性。
Thetcomplex spans 20 cM of the proximal region of mouse chromosome 17. A variant form, thethaplotype (t), exists at significant frequencies in wild mouse populations and is characterized by the presence of inversions that suppress recombination with wild-type (+) chromosomes. Transmission ratio distortion and sterility are associated withtand affect males only. It is hypothesized that these phenomena are caused bytrans-acting distorter/sterility factors that interact with a responder locus (Tcrt) and that the distorter and sterility factors are the same because homozygosity of the distorters causes male sterility. One factor,Tcd1, was previously shown to be amorphic using a chromosome deletion. To overcome limitations imposed by recombination suppression, we used a series of deletions within thetcomplexin transtotchromosomes to characterize theTcd1region. We find that the distorter activity ofTcd1is distinct from a linked sterility factor, originally calledtcs1.YACs mapped with respect to deletion breakpoints localizetcs1to a 1.1-Mb interval flanked byD17Aus9andTctex1.We present evidence for the existence of multiple proximaltcomplex regions that exhibit distorter activity. These studies demonstrate the utility of chromosome deletions for complex trait analysis.