Twirling reinforcing-reducing manipulation — central mechanism underlying antihypertensive effect on spontaneous hypertension in rats
Twirling reinforcing-reducing manipulation — central mechanism underlying antihypertensive effect on spontaneous hypertension in rats
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DOI：
10.1016/s0254-6272(18)30629-0
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发表时间：
2018-06
影响因子：
2.6
通讯作者：
Qiulei Guo;Qing-guo Liu;Dongmei Sun;Binbin Nie
中科院分区：
文献类型：
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作者：
Qiulei Guo;Qing-guo Liu;Dongmei Sun;Binbin Nie
目的研究旋回补泻手法(TRRM)对大鼠的降压作用，以确定其作用的中枢机制。方法采用18F-2-氟脱氧-D-葡萄糖正电子发射断层扫描(18F-FDG-PET)。56只自发性高血压大鼠(SHR)随机分为模型组、单针(SNA)组、旋补(SNA+TRF)组和旋消(SNA+TRD)组。14只Wistar大鼠设为对照组。针刺太冲穴干预SNA组、SNA+TRF组、SNA+TRD组，每日1次，间隔1d，共14d。所有大鼠在第14天重复测量血压并进行18F-FDG-PET显像。结果干预后SNA、SNA+TRF和SNA+TRD较模型组显著降低(P均<0.01)，SNA+TRF和SNA+TRD较SNA组均显著降低(P均<0.01)，其中SNA+TRD组降压效果最好(P<0.01)。TRRM激活的关键脑区主要集中在小脑、海马、下丘脑、延髓、岛叶皮质、中脑、丘脑和视皮层。结论TRRM通过改善激活关键脑区的脑糖代谢，显著降低SHR的血压，其中枢机制可能与中枢肾素-血管紧张素系统和神经递质有关。
OBJECTIVETo investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation (TRRM) might be the central mechanism underlying the action.METHODSIn the study,18F-2-fluoro-deoxy-D-glucose positron emission tomography (18F-FDG-PET) was employed. Fifity-six spontaneous hypertensive rats (SHRs) were randomly divided into a model group, a single-needle acupuncture (SNA) group, a twirling reinforcing group (SNA + TRF) and a twirling reducing (SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong (LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure (BP) of all rats was measured repeatedly and18F-FDG-PET scans were conducted on the 14th day. PET images were processed with Statistical Parametric Mapping 8.0.RESULTSAfter the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups (allP< 0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups (bothP< 0.01), with the SNA + TRD group exhibited the best antihypertensive effect (P< 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.CONCLUTIONTRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.