Generating mouse models for biomedical research: technological advances
Generating mouse models for biomedical research: technological advances
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DOI：
10.1242/dmm.029462
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Lloyd, Kevin C. Kent
中科院分区：
文献类型：
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作者：
Gurumurthy, Channabasavaiah B.;Lloyd, Kevin C. Kent
在过去的十年中，已经开发出新的方法和程序来产生人类疾病的基因工程小鼠模型。这篇概览文章强调了最近在小鼠基因组操作方面的几项技术进步，这些技术进步改变了我们在小鼠中操纵和研究基因表达的能力。我们讨论了通过胚胎干细胞同源重组的传统基因靶向如何让位于更精细的方法，使受精卵中的等位基因特异性操作成为可能。我们还强调了在使用可编程内切酶方面的进展，这些进展大大增加了编辑小鼠基因组的可行性和便利性。总之，这些技术和其他技术为研究人员提供了分子工具，以更高的保真度和特异性对小鼠基因组进行功能性注释，并使用更快、更简单和更便宜的技术生成新的小鼠模型。
Over the past decade, new methods and procedures have been developed to generate genetically engineered mouse models of human disease. This At a Glance article highlights several recent technical advances in mouse genome manipulation that have transformed our ability to manipulate and study gene expression in the mouse. We discuss how conventional gene targeting by homologous recombination in embryonic stem cells has given way to more refined methods that enable allele-specific manipulation in zygotes. We also highlight advances in the use of programmable endonucleases that have greatly increased the feasibility and ease of editing the mouse genome. Together, these and other technologies provide researchers with the molecular tools to functionally annotate the mouse genome with greater fidelity and specificity, as well as to generate new mouse models using faster, simpler and less costly techniques.