Inter- and intramolecular epitope spreading in equine recurrent uveitis
Inter- and intramolecular epitope spreading in equine recurrent uveitis
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DOI：
10.1167/iovs.05-0789
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Kaspers, B
中科院分区：
文献类型：
--
作者：
Deeg, CA;Amann, B;Kaspers, B
目的。检验马复发性葡萄膜炎自发模型中发生 S 抗原 (S-Ag) 和光感受器间视黄酸结合蛋白 (IRBP) 衍生表位的分子间和分子内扩散的假设。方法。将八匹患有马复发性葡萄膜炎（ERU）的马的免疫反应与五匹眼睛健康的对照马进行比较。每 8 周测试一次来自外周血 (PBL) 的淋巴细胞对 S-Ag 以及各种 S-Ag 和 IRBP 衍生肽的反应性，持续 12 至 39 个月（中位数为 22 个月）。在葡萄膜炎发作期间，分析了额外的血液样本。 结果。研究期间，所有 ERU 病例均检测到分子间表位扩散。七匹（八匹）患有 ERU 的马发生了分子内扩散。在观察期间分析了 14 例复发病例。复发期间，十次葡萄膜炎发作均伴有对 S-Ag 或 IRBP 衍生肽的新反应性。在没有任何炎症临床症状的情况下也可以检测到免疫反应谱的变化。眼睛健康的对照马在体外增殖测定中的所有时间点均为阴性。结论。在复发性葡萄膜炎自发模型中可检测到分子间和分子内扩散。免疫反应性的变化可以解释该疾病的缓解-复发特征。
PURPOSE. To test the hypothesis that inter- and intramolecular spreading to S-antigen (S-Ag) and interphotoreceptor retinoid binding protein (IRBP)-derived epitopes occurs in a spontaneous model of recurrent uveitis in the horse.METHODS. The immune response of eight horses with equine recurrent uveitis (ERU) was compared with that of five control horses with healthy eyes. Lymphocytes derived from peripheral blood (PBLs) were tested every 8 weeks for their reactivity against S-Ag and various S-Ag and IRBP-derived peptides for 12 to 39 months (median, 22 months). During uveitic episodes, additional blood samples were analyzed.RESULTS. Intermolecular epitope spreading was detectable in all ERU cases during the study. Intramolecular spreading occurred in seven (of eight) horses with ERU. Fourteen relapses were analyzed during the observation period. Ten uveitic episodes were accompanied by neoreactivity to S-Ag or IRBP-derived peptides during the relapse. Shifts in the immune response profile were also detectable without any clinical signs of inflammation. Eye-healthy control horses were negative at all time points in the in vitro proliferation assays.CONCLUSIONS. Inter and intramolecular spreading was detectable in a spontaneous model of recurrent uveitis. The shifts in immunoreactivity could account for the remitting - relapsing character of the disease.