Transmembrane Helices Tilt, Bend, Slide, Torque, and Unwind between Functional States of Rhodopsin.
Transmembrane Helices Tilt, Bend, Slide, Torque, and Unwind between Functional States of Rhodopsin.
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DOI：
10.1038/srep34129
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发表时间：
2016-09-23
影响因子：
4.6
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Ren Z;Ren PX;Balusu R;Yang X
当跨膜受体蛋白被激活时，视紫红质和其他g蛋白偶联受体的七螺旋束经历结构重排。已知这些结构变化涉及各种跨膜螺旋的倾斜和弯曲。然而，导致g蛋白结合细胞质裂缝的结构事件之间的因果关系尚不清楚。在这里，我们提出了一个蛋白质螺旋的数学模型和一个简单的程序，以确定多个参数，提供螺旋构象的精确描述。对牛视紫红质结构的全面调查表明，在视紫红质的激活过程中，螺旋重排涉及各种角度和线性运动，如扭转、解绕和滑动，除了先前报道的倾斜和弯曲。这些迄今为止未定义的运动成分统一了从不同实验方法获得的结果，并证明了活性视蛋白结构与视网膜锚点附近晶体中的光活化结构之间的构象相似性，尽管它们存在显着差异。
The seven-helical bundle of rhodopsin and other G-protein coupled receptors undergoes structural rearrangements as the transmembrane receptor protein is activated. These structural changes are known to involve tilting and bending of various transmembrane helices. However, the cause and effect relationship among structural events leading to a cytoplasmic crevasse for G-protein binding is less well defined. Here we present a mathematical model of the protein helix and a simple procedure to determine multiple parameters that offer precise depiction of a helical conformation. A comprehensive survey of bovine rhodopsin structures shows that the helical rearrangements during the activation of rhodopsin involve a variety of angular and linear motions such as torsion, unwinding, and sliding in addition to the previously reported tilting and bending. These hitherto undefined motion components unify the results obtained from different experimental approaches, and demonstrate conformational similarity between the active opsin structure and the photoactivated structures in crystallo near the retinal anchor despite their marked differences.
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影响因子：
64.8
作者：
Scheerer, Patrick;Park, Jung Hee;Ernst, Oliver P.
通讯作者：
Ernst, Oliver P.
影响因子：
2.1
作者：
Aravind, L;Ponting, CP
通讯作者：
Ponting, CP
DOI：
10.1073/pnas.0608022103
发表时间：
2006-10-31
影响因子：
11.1
作者：
Salom, David;Lodowski, David T.;Palczewski, Krzysztof
通讯作者：
Palczewski, Krzysztof
影响因子：
3
作者：
Ferris, Hedda U.;Zeth, Kornelius;Lupas, Andrei N.
通讯作者：
Lupas, Andrei N.
DOI：
10.1016/j.str.2010.01.013
发表时间：
2010-03-14
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Airola MV;Watts KJ;Bilwes AM;Crane BR
通讯作者：
Crane BR