Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription
Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription
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DOI：
10.1016/j.molcel.2005.05.009
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发表时间：
2005-06-10
期刊：
影响因子：
16
通讯作者：
Mellor, J
中科院分区：
文献类型：
--
作者：
Morillon, A;Karabetsou, N;Mellor, J
共价修饰是控制基因转录的关键表观遗传标记。组蛋白H3上的多个赖氨酸残基甲基化（me），但其功能尚不清楚。在这里，我们展示了在酵母MET16转录诱导过程中组合和动态H3甲基化的两个阶段。K4me3与K36me2/3定义了起始后调控期，在转录延伸开始时，K4me2与K79me2出现之前。Isw1 atp酶延迟起始RNA聚合酶II （RNAPII）的释放进入延伸，以促进染色质修饰。与Set1 （COMPASS）和Set2相关的复合物的Spp1亚基，分别决定K4me3和K36me2/3，是瞬时依赖nua4的H4K8ac所必需的。这将RNAPII从Isw1控制中释放出来，并促进受控的转录延伸和终止。我们认为新启动的RNAPII受表观遗传控制。
Covalent modifications to histories are key epigenetic marks that control gene transcription. Multiple lysine residues on histone H3 are methylated (me), but their functions are unclear. Here, we demonstrate two phases of combinatorial and dynamic H3 methylation during induction of transcription at MET16 in yeast. K4me3 with K36me2/3 define a postinitiation regulatory phase and precede the appearance of K4me2 with K79me2 at the onset of transcript elongation. The Isw1 ATPase delays the release of initiated RNA polymerase II (RNAPII) into elongation to facilitate chromatin modifications. The Spp1 subunit of complex associated with Set1 (COMPASS) and Set2, determining K4me3 and K36me2/3, respectively, are required for transient NuA4-dependent H4K8ac. This releases RNAPII from Isw1 control and promotes controlled transcription elongation and termination. We propose that newly initiated RNAPII is under epigenetic control.