Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions by "in vivo cryotechnique"
Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions by "in vivo cryotechnique"
复制标题
DOI：
10.1007/s00418-006-0175-4
复制
发表时间：
2006-09-01
影响因子：
2.3
通讯作者：
Ohno, Shinichi
中科院分区：
文献类型：
--
作者：
Li, Zilong;Ohno, Nobuhiko;Ohno, Shinichi
血清蛋白在肾小球中的分布是医学和生物学领域的重要病理组织学研究内容，但其通过肾小球毛细血管袢（GCL）的机制尚不清楚。我们试图通过“体内冷冻技术”结合免疫组织化学观察血清蛋白通过GCL的地形变化。正常血压下，白蛋白、IgG、IG kappa轻链和IgG 1重链主要分布于GCL，而不与ZO-1共定位。在心脏停搏条件下和速冻新鲜组织中，白蛋白和κ轻链免疫定位于Bowman间隙，表明它们是由于血液供应中断而通过的。然而，在急性高血压条件下，它们更清楚地免疫定位沿着基底膜和在Bowman的空间，表明他们增加通过GCL。IgG也更清楚地定位于系膜区急性高血压，与正常血压或心脏骤停的条件下相比。这项研究是第一次直接可视化肾小球通过血清蛋白异常血流动力学条件下的“在体内冷冻技术”，实验方案将是有用的形态功能检查活小鼠GCL和免疫组化分析的动态变化的蛋白质。
Distribution of serum proteins in renal glomeruli is important for histopathology in medical and biological fields, but mechanisms of their passage through glomerular capillary loops (GCL) are still difficult to clarify. We have tried to visualize topographical changes of the serum proteins passing through GCL by "in vivo cryotechnique" in combination with immunohistochemistry. Albumin and immunoglobulin G (IgG), Ig kappa light chain and IgG1 heavy chain were mainly immunolocalized in GCL, but not colocalized with zonula occludens-1 (ZO-1) under normotensive condition. Under heart-arrest condition and in quick-frozen fresh tissues, albumin and kappa light chain were immunolocalized in Bowman's space, indicating their passage caused by the stoppage of blood supply. However, under acute hypertensive condition, they were more clearly immunolocalized along basement membranes and in the Bowman's space, indicating their increased passage through GCL. IgG was also more clearly localized in mesangial areas under acute hypertension, compared with that under the normotensive or heart-arrest condition. This study is the first direct visualization for glomerular passage of serum proteins under abnormal hemodynamic conditions by the "in vivo cryotechnique", and the experimental protocol will be useful for morphofunctional examination of living mouse GCL and immunohistochemical analyses of dynamically changing proteins.