Determination of cortical bone porosity and pore size distribution using a low field pulsed NMR approach
Determination of cortical bone porosity and pore size distribution using a low field pulsed NMR approach
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DOI：
10.1016/s0736-0266(02)00157-2
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发表时间：
2003-03-01
影响因子：
2.8
通讯作者：
Ni, QW
中科院分区：
文献类型：
--
作者：
Wang, XD;Ni, QW
本研究的目的是首先证明低场脉冲核磁共振（NMR）过程的概念，用于评估体外人骨的皮质孔隙率和孔径分布，然后应用该技术检测这些参数中与年龄相关的骨变化。使用Carr-Pureell-Meiboom-Gill NMR自旋回波链方法来确定孔隙率，并使用反转NMR自旋-自旋弛豫（T-2）谱来评估皮质骨中的孔径分布。使用这些技术，皮质骨孔隙度和孔径分布的19个标本的人尸骨，从16岁到89岁，进行了评估。将NMR结果与相同骨样本的组织形态计量学数据进行比较，以验证NMR方法的有效性。此外，一个系数（表面弛豫率）的孔径的T-2弛豫时间的经验确定的哈弗氏管和骨细胞陷窝。本研究的结果表明，在体外核磁共振的方法，使用T-2弛豫技术可以直接评估的孔隙率和孔径分布（哈佛管和骨细胞陷窝）在人皮质骨。此外，这项研究表明，年龄相关的变化，在皮质孔隙度主要涉及哈佛管，而骨细胞陷窝的孔隙度似乎是独立的年龄。(C)2003骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
The objective of this study was first to prove the concept of a low field pulsed nuclear magnetic resonance (NMR) process for assessing the cortical porosity and pore size distribution of human bone in vitro, and then to apply the technique to detect age-related changes of bone in these parameters. The Carr-Pureell-Meiboom-Gill NMR spin echo train method is used to determine the porosity, and an inversion NMR spin-spin relaxation (T-2) spectrum is used to assess the pore size distribution in cortical bone. Using these techniques, cortical porosity and pore size distribution of 19 specimens of human cadaveric bone, ranging from 16 to 89 years of age, were assessed. The NMR results were compared with the histomorphometric data of the same bone samples to verify the efficacy of the NMR approach. Moreover, a coefficient (surface relaxivity) relating the pore size to the T-2 relaxation time was determined empirically tor the Haversian canals and the osteocytic lacunae. The results of this study demonstrate that the in vitro NMR approach using T-2 relaxation techniques can directly assess the porosity and pore size distribution (Haversian canals and osteocytic lacunae) in human cortical bone. In addition, this study indicates that the age-related changes in cortical porosity relate predominantly to Haversian canals, whereas the porosity of osteocytic lacunae appears to be independent of age. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.