The central clock controls the daily rhythm of Aqp5 expression in salivary glands
The central clock controls the daily rhythm of Aqp5 expression in salivary glands
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DOI：
10.1007/s12576-017-0540-1
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发表时间：
2018-07-01
影响因子：
2.3
通讯作者：
Nakamura, Wataru
中科院分区：
文献类型：
--
作者：
Uchida, Hitoshi;Nakamura, Takahiro J.;Nakamura, Wataru
唾液分泌表现出昼夜变化，由生物钟控制。视交叉上核(SCN)的中枢时钟通过促使外周振荡器适应不断变化的环境来调节日常生理节律。已知水通道蛋白5(AQP5)在唾液分泌中起关键作用，但AQP5与昼夜节律之间的关系却知之甚少。我们研究的目的是评估AQP5在颌下腺(SMGs)的表达是由SCN的中枢时钟驱动的还是由自主振荡驱动的。我们观察到在培养的SMG中，周期昼夜节律蛋白Homolog 2和荧光素酶融合蛋白(PER2：：Luc)的活性存在昼夜节律波动，其周期性依赖于核心时钟基因。在体内，AQP5在SMGs中的表达呈昼夜节律。在体外培养的SMGs中，Clock基因表现出明显的昼夜节律，而AQP5的表达则没有。这些数据表明，小鼠SMG中AQP5的日常表达是由SCN的中枢时钟驱动的。
Salivary secretion displays day-night variations that are controlled by the circadian clock. The central clock in the suprachiasmatic nucleus (SCN) regulates daily physiological rhythms by prompting peripheral oscillators to adjust to changing environments. Aquaporin 5 (Aqp5) is known to play a key role in salivary secretion, but the association between Aqp5 and the circadian rhythm is poorly understood. The aim of our study was to evaluate whether Aqp5 expression in submandibular glands (SMGs) is driven by the central clock in the SCN or by autonomous oscillations. We observed circadian oscillations in the activity of period circadian protein homolog 2 and luciferase fusion protein (PER2::LUC) in cultured SMGs with periodicity depending on core clock genes. A daily rhythm was detected in the expression profiles of Aqp5 in SMGs in vivo. In cultured SMGs ex vivo, clock genes showed distinct circadian rhythms, whereas Aqp5 expression did not. These data indicate that daily Aqp5 expression in the mouse SMG is driven by the central clock in the SCN.