Interaction of tomato yellow leaf curl virus with diverse betasatellites enhances symptom severity
Interaction of tomato yellow leaf curl virus with diverse betasatellites enhances symptom severity
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DOI：
10.1007/s00705-009-0431-8
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发表时间：
2009-08-01
影响因子：
2.7
通讯作者：
Ikegami, Masato
中科院分区：
文献类型：
--
作者：
Ito, Takuya;Kimbara, Junji;Ikegami, Masato
测定了一株发生卷叶和黄化症状的日本赤木县番茄上分离的卷叶病毒的全核苷酸序列。该病毒的基因组结构与其他东半球的单分体海豚病毒相似。既没有检测到DNAβ卫星，也没有检测到DNA-B成分。与番茄黄化曲叶病毒以色列[Japan：TOSA：2005](TYLCV-IL[JR：TOS：05])和TYLCV-以色列[Japan：Haruno：2005](TYLCV-IL[JR：HAN：05])的核苷酸序列同源性最高(99%)。其外壳蛋白V1与TYLCV-IL[JR：TOS：05]和TYLCVIL[JR：HAN：05]具有相同的氨基酸序列。因此，该巨细胞病毒被确定为TYLCV-IL的一个分离物，命名为TYLCV以色列[Japan：Tochigi：2007](TYLCV-IL[JR：TOC：07])。我们研究了TYLCV-IL[JR：TOC：07]与两个已知的与日本番茄黄矮病相关的卫星[Japan：Ibaraki：2006]和金银花黄脉花叶卫星[Japan：Nara：2006]以及与番茄曲叶菲律宾卫星[菲律宾：Laguna1：2008]在番茄和烟草上的互作。TYLCVIL[JR：TOC：07]反式复制了这些Betasellite，比单独使用TYLCV-IL[JR：TOC：07]诱导了更严重的番茄黄化卷叶病相关症状。
The complete nucleotide sequence was determined for a begomovirus isolated from tomato exhibiting leaf curling and yellowing symptoms in Tochigi Prefecture in Japan. The genome organization of this virus was similar to those of other Old World monopartite begomoviruses. Neither a DNA betasatellite nor a DNA-B component was detected. It had the highest total nucleotide sequence identity (99%) with tomato yellow leaf curl virus-Israel [Japan: Tosa: 2005] (TYLCV-IL[JR:Tos:05]) and TYLCV-Israel[Japan: Haruno: 2005] (TYLCV-IL[JR:Han:05]). Its coat protein V1 also showed an identical amino acid sequence with those of TYLCV-IL[JR: Tos: 05] and TYLCVIL[JR: Han: 05]. Thus, the begomovirus was determined to be an isolate of TYLCV-IL designated as TYLCVIsrael[Japan: Tochigi: 2007] (TYLCV-IL[JR:Toc:07]). We investigated the interaction of TYLCV-IL[JR: Toc: 07] with two known satellites associated with tomato yellow dwarf disease in Japan, tobacco leaf curl Japan betasatellite [Japan: Ibaraki: 2006] and honeysuckle yellow vein mosaic betasatellite [Japan: Nara: 2006], as well as with tomato leaf curl Philippines betasatellite [Philippines: Laguna1:2008], in tomato and Nicotiana benthamiana plants. TYLCVIL[JR: Toc: 07] trans-replicated these betasatellites, inducing more severe tomato yellow leaf curl disease-related symptoms than TYLCV- IL[JR:Toc:07] alone.