Cloning and sequence analysis of a cDNA clone from Arabidopsis thaliana homologous to a proteasome alpha subunit from Drosophila.
Cloning and sequence analysis of a cDNA clone from Arabidopsis thaliana homologous to a proteasome alpha subunit from Drosophila.
复制标题
与果蝇蛋白酶体 α 亚基同源的拟南芥 cDNA 克隆的克隆和序列分析。
作者：
P. Genschik;G. Philipps;C. Gigot;J. Fleck
从拟南芥细胞悬浮培养文库中分离的cDNA克隆显示出与果蝇的蛋白酶体28 kDa α亚基相当大的相似性[（1990）Gene 90，235-241]。拟南芥TAS-g64克隆编码的250个氨基酸长的蛋白质在其一级结构和预测的二级结构上与果蝇的PROS-28.1克隆具有重要的同源性。在两个序列之间观察到的唯一差异是20个C-末端氨基酸。该亚基可能在两个王国中共享重要功能，正如植物和动物之间的重要保护所揭示的那样。在植物细胞中，它由单拷贝基因编码，可能受胁迫和/或分裂的调节。
A cDNA clone isolated from an Arabidopsis thaliana cell suspension culture library showed considerable similarities to the proteasome 28 kDa alpha subunit of Drosophila [(1990) Gene 90, 235-241]. The 250 amino acid-long protein encoded by Arabidopsis TAS-g64 clone has important homologies in its primary structure and in the predicted secondary structure with the PROS-28.1 clone from Drosophila. The only divergence observed between the two sequences is for the 20 C-terminal amino acids. This subunit might share important functions in both kingdoms, as revealed by the important conservation between plants and animals. In plant cells it is encoded by a single-copy gene and probably regulated by stress and/of division.