Endodontic pathogens stimulate monocyte chemoattractant protein-1 and interleukin-8 in mononuclear cells
Endodontic pathogens stimulate monocyte chemoattractant protein-1 and interleukin-8 in mononuclear cells
复制标题
DOI：
10.1016/s0099-2399(98)80083-6
复制
发表时间：
1998-02-01
影响因子：
4.2
通讯作者：
Graves, DT
中科院分区：
文献类型：
--
作者：
Jiang, YL;Russell, TR;Graves, DT
牙髓的微生物感染导致白细胞的募集和牙髓源性病变的形成。趋化因子白细胞介素-8（IL-8）和单核细胞趋化蛋白-1（MCP-1）分别是中性粒细胞和单核细胞的相对特异性趋化剂。在目前的研究中，外周血单核细胞刺激链球菌突变体，牙髓卟啉单胞菌，厌氧消化链球菌，这是与病变的牙髓起源。通过ELISA测定，这些细菌中的每一种诱导IL-8和MCP-1的剂量依赖性增加。诱导的水平是生理相关的。然而，与S.突变体或厌氧假单胞菌。因此，这些细菌可以诱导显著水平的趋化因子IL-8和MCP-1，这可能有助于体内嗜中性粒细胞或单核细胞的募集。这些介质的表达可能有助于牙髓感染的发展，特别是在炎症性白细胞募集方面。
Microbial infection of the dental pulp leads to the recruitment of leukocytes and the formation of lesions of endodontic origin. The chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are relatively specific chemoattractants for neutrophils and monocytes, respectively. In the present studies, peripheral blood mononuclear cells were stimulated by Streptococcus mutants, Porphyromonas endodontalis, and Peptostreptococcus anaerobius, which are associated with lesions of endodontic origin. Each of these bacteria induced a dose-dependent increase in IL-8 and MCP-1, determined by ELISA. The levels induced are physiologically relevant. However, low doses of P. endodontalis were less effective in inducing IL-8 or MCP-1 expression, compared with S. mutants or P. anaerobius. Thus, these bacteria can induce significant levels of the chemokines IL-8 and MCP-1, which could contribute to the recruitment of neutrophils or monocytes in vivo. The expression of these mediators may contribute to the development of endodontic infections, particularly with regard to inflammatory leukocyte recruitment.