Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles
Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles
复制标题
DOI：
10.1073/pnas.97.11.6120
复制
发表时间：
2000-05-23
影响因子：
11.1
通讯作者：
Llin치s, R
中科院分区：
文献类型：
--
作者：
Daly, C;Sugimori, M;Llin치s, R
GTP酶Dynamin I是突触小泡(SV)内吞所必需的，我们观察到Dynamin以一种钙离子依赖的方式与SV蛋白突触素结合，这表明Dynamin/Synaptophysin复合体在SV循环中发挥作用的可能性。在本文中，我们证明了多肽注射到鱿鱼巨型突触前终末破坏Dynamin/Synaptophysin相互作用导致高频刺激时递质释放减少，表明SV循环受到抑制。我们证明了融合后囊泡回收的阻断，此外，我们观察到笼蛋白包裹的小泡增加，表明该肽不阻止依赖于笼蛋白的内吞作用，我们得出结论，动力蛋白/突触素复合体在有效的突触传递所需的非笼蛋白依赖的SV内吞作用机制中发挥作用。
The GTPase dynamin I is required for synaptic vesicle (SV) endocytosis, Our observation that dynamin binds to the SV protein synaptophysin in a Ca2+-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling, In this paper we show that disruption of the dynamin/synaptophysin interaction by peptide injection into the squid giant synapse preterminal results in a decrease in transmitter release during high-frequency stimulation, indicating an inhibition of SV recycling, Electron microscopy of these synapses reveals a depletion of SVs. demonstrating a block of vesicle retrieval after fusion, In addition, we observed an increase in clathrin-coated vesicles, indicating that the peptide does not block clathrin-dependent endocytosis, We conclude that the dynamin/synaptophysin complex functions in a clathrin-independent mechanism of SV endocytosis that is required for efficient synaptic transmission.