Pandrug-resistant isolate of Klebsiella pneumoniae causes less damage than drug-susceptible isolates in a rabbit model.
Pandrug-resistant isolate of Klebsiella pneumoniae causes less damage than drug-susceptible isolates in a rabbit model.
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DOI：
10.25011/cim.v34i1.14911
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
Zheng Zhou;Jianan Ren;Haiyan Liu;G. Gu;Jie‐shou Li
中科院分区：
文献类型：
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作者：
Zheng Zhou;Jianan Ren;Haiyan Liu;G. Gu;Jie‐shou Li
细菌感染引起一系列炎症反应，导致住院时间延长和死亡率增加。在临床工作中，我们经常发现由药物敏感分离株引起的感染比由泛耐药分离株引起的感染具有更差的结局。本研究的目的是评估耐药性在肺炎克雷伯菌感染的兔模型中的影响。方法采用家兔实验性菌血症模型，分别用敏感（A）、多重耐药（B）和泛耐药（C）肺炎克雷伯菌进行攻毒。测定最低抑菌浓度（MIC）、白细胞、TNFα、IL-17和HMGB-1水平以及存活时间。结果A、B和C菌株攻毒后的平均存活时间分别为10.5 ± 3.63、12.7 ± 2.31和13.9 ± 0.32天。与分离株A攻毒后的白细胞水平相比，分离株C攻毒后的白细胞水平较低（p = 0.002）。与分离株B或C相比，分离株A攻毒组的致病病原体血细胞计数和TNFα、IL-17和HMGB-1浓度较高。动物死亡后，A组的组织细菌负荷显著高于分离株B和C。结论泛耐药菌引起的菌血症较敏感菌引起的菌血症危害小。感染泛耐药克雷伯氏菌的家兔。与感染药物敏感分离株的动物相比，感染肺炎链球菌的动物存活时间更长，炎症反应更低。
PURPOSE Bacterial infections induce a series of inflammatory responses and lead to longer hospital stays and increased mortality. In clinical work, we often find that infections caused by drug-susceptible isolates have a worse outcome than those caused by pandrug-resistant isolates. To goal of this study was to assess the impact of drug resistant in a rabbit model of Klebsiella pneumoniae infection. METHODS This study used a rabbit model of experimental bacteremia, challenged by susceptible (A), multidrug-resistant (B) and pandrug-resistant (C) isolates of Klebsiella pneumoniae. Mimimal inhibitory concentrations (MIC), leukocyte, TNFα, IL-17, and HMGB-1 levels and survival times were measured. RESULTS Mean survival times after challenge by isolates A, B and C were 10.5 ± 3.63, 12.7 ± 2.31 and 13.9 ± 0.32 days, respectively. Leukocytes levels after challenge with isolate C were lower compared with those after challenge with isolate A (p = 0.002). Blood counts of the offending pathogens and concentrations of TNFα, IL-17, and HMGB-1 were higher in the group challenged by isolate A in comparison with isolate B or C. Tissue bacterial load after animal death was significantly higher in rabbits of group A in comparison with isolates B and C. CONCLUSION Bacteremia induced by pandrug-resistant isolates is accompanied by less damage compared with bacteremia by drug-susceptible isolates. Rabbits infected with a pandrug-resistant isolate of K. pneumoniae survived longer and had a lower inflammatory response than did animals infected with drug-susceptible isolates.