Intermittent fasting modulation of the diabetic syndrome in sand rats. III. Post-mortem investigations
Intermittent fasting modulation of the diabetic syndrome in sand rats. III. Post-mortem investigations
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DOI：
10.3892/ijmm.2010.556
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发表时间：
2011-01-01
影响因子：
5.4
通讯作者：
Malaisse, Willy J.
中科院分区：
文献类型：
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作者：
Belkacemi, Louiza;Selselet-Attou, Ghalem;Malaisse, Willy J.
本报告涉及在沙鼠身上检查的几个死后变量，这些沙鼠要么保持素食(对照动物)，要么在20天的过渡期内首先暴露于由固定数量的高热量食物和减少数量的植物食物组成的混合饮食，然后暴露在30天的高热量食物暴露的试验期。在后一阶段，所有动物要么自由进食，要么每天禁食15小时，即从下午5点开始。至上午8时测定体重、肝脏湿重、胰腺湿重、血糖和血红蛋白A1c浓度、血浆胰岛素浓度、胰岛素分泌指数、胰岛素抵抗指数、血浆胆固醇和甘油三酯浓度、肝脏甘油三酯和磷脂含量。胰岛(胰岛素，GLUT2)和肝脏(脂滴)组织学检查。主要发现禁食动物的体重低于非禁食动物，非糖尿病和糖尿病大鼠的肝脏重量高于非禁食动物(但禁食动物不是这样)，非糖尿病动物和糖尿病大鼠的胰腺重量与对照动物不同，禁食导致血糖、血浆胰岛素和胰岛素抵抗指数、血浆胆固醇和甘油三酯浓度以及甘油三酯肝脏含量下降。
The present report concerns several post-mortem variables examined in sand rats that were either maintained on a vegetal diet (control animals) or exposed first during a 20-day transition period to a mixed diet consisting of a fixed amount of a hypercaloric food and decreasing amounts of the vegetal food and then to a 30-day experimental period of exposure to the hypercaloric food. During the latter period, all animals were either given free access to food or fasting daily for 15 h, i.e. from 5.00 p.m. to 8.00 a.m. The body weight, liver wet weight, pancreas wet weight, plasma glucose and haemoglobin A1c concentration, plasma insulin concentration, insulinogenic index, insulin resistance HOMA, plasma cholesterol and triglyceride concentration, liver triglyceride and phospholipid content were all measured. Pancreatic islet (insulin, GLUT2) and liver (lipid droplets) histology were also examined. The main findings consisted in a lower body weight of fasting than non-fasting animals, a higher liver weight in non-diabetic and diabetic rats than in control non-fasting (but not so in fasting) animals, a decrease of pancreas weight in non-diabetic and diabetic as distinct from control animals, a fasting-induced decrease in plasma glucose, plasma insulin and insulin resistance HOMA, plasma cholesterol and triglyceride concentration and triglyceride liver content.