Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)
Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)
复制标题
稀有米诺鱼 (Gobiocypris rarus) 生殖细胞特异性表达死端 (dnd)
DOI：
10.1007/s10695-015-0029-x
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发表时间：
2015-04-01
影响因子：
2.9
通讯作者：
Zhao, Haobin
中科院分区：
文献类型：
--
作者：
Duan, Jundan;Feng, Gongqing;Zhao, Haobin
稀有鲦鱼（Gobiocypris rarus）是中国一种新兴的模式鱼，其性腺的发育尚不清楚。生殖细胞特异性基因在动物体内是保守的。死端（Dead end, Dnd）作为一种细菌颗粒成分在斑马鱼体内首次被发现。在这里，我们报道了dnd在稀有小鱼中的克隆和表达谱。RT-PCR结果显示，dnd在两性性腺中特异性表达，来源于母体，在胚胎发生过程中持续表达。Dnd mRNA仅在睾丸和卵巢生殖细胞中检测到。在斑马鱼胚胎发生过程中，dnd mRNA的时间表达与vasa和dnd相似。综上所述，dnd和mRNA仅存在于罕见的小鱼的生殖细胞中。
Rare minnow (Gobiocypris rarus) is an emerging model fish in China, and the development of its gonads is still elusive. Germ cell-specific genes are conserved in animals. Dead end (Dnd) was first documented as a germ granule component in zebrafish. Here, we report the cloning and expression profile of dnd in rare minnow. RT-PCR results showed that dnd is expressed specifically in the gonads of both sexes, is maternal in origin and is expressed continuously during embryogenesis. Dnd mRNA could be detected exclusively in the germ cells of the testis and ovary. Temporal expression of dnd mRNA is similar to that of vasa and dnd in zebrafish during embryogenesis. Taken together, dnd mRNA is restricted to the germ cells of rare minnow.