The Vroman effect: Competitive protein exchange with dynamic multilayer protein aggregates
The Vroman effect: Competitive protein exchange with dynamic multilayer protein aggregates
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DOI：
10.1016/j.colsurfb.2012.10.039
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发表时间：
2013-03-01
影响因子：
5.8
通讯作者：
Bilek, Marcela M. M.
中科院分区：
文献类型：
--
作者：
Hirsh, Stacey L.;McKenzie, David R.;Bilek, Marcela M. M.
蛋白质的表面固定化是一个新兴的领域，在广泛的重要领域中具有应用：生物医学设备、疾病诊断、生物传感、食品加工、生物污垢和生物反应器。在自然界中，蛋白质通常协同工作，如在重要的酶混合物纤维素酶中。当利用表面固定化蛋白质时，有必要保持这些协同作用。然而，较早吸附的蛋白质被具有较强结合亲和力的其他蛋白质竞争性置换（“Vroman效应”）导致难以控制的不期望的层不稳定性。虽然这种纳米级的现象在过去的40年里得到了广泛的研究，但这种竞争性交换发生的过程还没有得到很好的理解。本文使用原子力显微镜，QCM-D，TOF-SIMS和溶液中的TOF-MS表明，这种竞争性交换过程可以通过多层蛋白质聚集体的转向发生。这种动态过程与早期假设的“瞬时复合物”模型一致，其中交换发生在三个阶段：初始层吸附，另一个蛋白质层然后将其自身嵌入初始层，形成“瞬时复合物”：复合物“转向”，将第一层暴露于溶液;来自第一层的蛋白质解吸，导致最终吸附的蛋白质组合物富含来自第二层的蛋白质。(C)2012爱思唯尔有限公司版权所有。
The surface immobilization of proteins is an emerging field with applications in a wide range of important areas: biomedical devices, disease diagnosis, biosensing, food processing, biofouling, and bioreactors. Proteins, in Nature, often work synergistically, as in the important enzyme mixture, cellulase. It is necessary to preserve these synergies when utilizing surface immobilized proteins. However, the competitive displacement of earlier adsorbed proteins by other proteins with stronger binding affinities (the "Vroman effect") results in undesired layer instabilities that are difficult to control. Although this nanoscale phenomenon has been extensively studied over the last 40 years, the process through which this competitive exchange occurs is not well understood. This paper uses atomic force microscopy, QCM-D, TOF-SIMS, and in-solution TOF-MS to show that this competitive exchange process can occur through the turning of multilayer protein aggregates. This dynamic process is consistent with earlier postulated "transient complex" models, in which the exchange occurs in three stages: an initial layer adsorbs, another protein layer then embeds itself into the initial layer, forming a "transient complex:" the complex "turns," exposing the first layer to solution; proteins from the first layer desorb resulting in a final adsorbed protein composition that is enriched in proteins from the second layer. (C) 2012 Elsevier B.V. All rights reserved.