Change in Morphology of Dimyristoylphosphatidylcholine/Bile Salt Derivative Bicelle Assemblies with Dodecylmaltoside in the Disk and Ribbon Phases
Change in Morphology of Dimyristoylphosphatidylcholine/Bile Salt Derivative Bicelle Assemblies with Dodecylmaltoside in the Disk and Ribbon Phases
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十二烷基麦芽糖苷在盘相和带相中二肉豆蔻酰磷脂酰胆碱/胆盐衍生物 Bicelle 组件的形态变化
DOI：
10.1021/acs.jpclett.2c02445
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Urban, Volker S.
中科院分区：
文献类型：
--
作者：
Leite, Wellington C.;Wu, Yuqi;Pingali, Sai Venkatesh;Lieberman, Raquel L.;Urban, Volker S.
双胞由长链和短链脂质的混合物组成，形成纳米结构的分子组装体，是有吸引力的脂质-膜模拟物，用于完整膜蛋白的体外研究。本文研究了第三种组分--单链去污剂十二烷基-β-d-麦芽糖苷（DDM）对1，2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）和3-[（3-胆酰胺丙基）二甲氨基]-2-羟基-1-丙磺酸盐（CHAPSO）组成的胶束在低于（10 °C）和高于（38 °C）相变时形态的影响。在不存在DDM的情况下，双胞在10 °C下从椭圆形盘转化为在38 °C下延伸的带状结构。DDM的加入使椭圆形的盘形物变为圆形，使扁平的带状物变为圆柱形的蠕虫状胶束。单链洗涤剂DDM对双胞纳米级形态的影响的知识有助于理解脂质膜自组织和优化膜生物学研究的脂质模拟物的目标。
Bicelles, composed of a mixture of long and short chain lipids, form nanostructured molecular assemblies that are attractive lipid-membrane mimics forin vitrostudies of integral membrane proteins. Here we study the effect of a third component, the single chain detergentn-dodecyl-β-d-maltoside (DDM) on the morphology of bicelles composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO) below (10 °C) and above (38 °C) the phase transition. In the absence of DDM, bicelles convert from ellipsoidal disks at 10 °C to extended ribbon-like structures at 38 °C. The addition of DDM reshapes the ellipsoidal disc to a circular one and the flattened ribbon to a circular-cylinder worm-like micelle. Knowledge of the influence of the single chain detergent DDM on bicelle nanoscale morphology contributes toward comprehending lipid membrane self-organization and to the goal of optimizing lipid mimics for membrane biology research.