New insight into waste activated sludge acetogenesis triggered by coupling sulfite/ferrate oxidation with sulfate reduction-mediated syntrophic consortia
New insight into waste activated sludge acetogenesis triggered by coupling sulfite/ferrate oxidation with sulfate reduction-mediated syntrophic consortia
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对亚硫酸盐/高铁酸盐氧化与硫酸盐还原介导的互养聚生体耦合引发的废弃活性污泥乙酸生成的新见解
DOI：
10.1016/j.cej.2020.125885
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发表时间：
2020-11
影响因子：
15.1
通讯作者：
Yue Xiuping
中科院分区：
文献类型：
--
作者：
Zhou Aijuan;Liu Hongyan;Varrone Cristiano;Shyryn Alimzhanova;Defemur Zafiry;Wang Sufang;Liu Wenzong;Yue Xiuping
乙酸乙酯(HAC)是一种很有前途的活性污泥生物精制方法，但3-5碳原子短链脂肪酸(SCFAs)的生物转化热力学限制了该方法的应用。以硫酸根(SO_4-∙‾)为基础的高级氧化被认为是加速发酵的合适候选者。在本研究中，我们富集了一株不完全氧化的硫酸盐还原细菌(io-sRb)，并结合高铁酸钾(PF)和亚硫酸钠(Na_2SO_3)产生的SO_4-∙‾氧化作用，促进了乙酰化反应的发生。SO_4SRB氧化生成的硫酸盐是IO-∙‾代谢的必需底物。给出了基于实验数据的全过程概念验证。结果证实，PF+Na2SO3+SRB试验的SCFAs产生量最高(4261±2210 mg COD/L，HAc为60.9±20.5%)，高于未经io-SRB调节的PF+Na2SO3试验(2521±2109 mg COD/L，HAc为50.6±60.3%)。粒度分析和荧光光谱分析表明，PF+和Na2SO3氧化对可溶性有机物的释放有促进作用。电子顺磁共振和自由基清除分析表明，SO4-∙‾是最关键的自由基，起着最重要的作用。X-射线光电子能谱分析表明，io-sRb能进一步促进多糖和蛋白质在SO4-∙‾氧化的基础上转化为羧酸。此外，79%的Fe(VI)被还原为Fe(III)，大部分S(IV)被转化为SO42SRB，其中约40%被IO-−联合体代谢。显然，SO4SRB氧化和io-∙‾刺激显著改变了关键微生物组的组成，其中发酵产酸菌占主导地位。通过分子生态网络分析，揭示了io-SRB、水解菌和产酸菌之间可能存在的协同关系。本研究为提高SO_4-∙−发酵生物代谢产物的回收率提供了新的思路。
Acetate (HAc) production via acetogenesis is a promising biorefinery approach for waste activated sludge (WAS); however, it is hampered by the thermodynamic constraints of the bioconversion of 3–5 carbon atom short-chain fatty acids (SCFAs). Sulfate radical (SO4∙‾)-based advanced oxidation is regarded as an appropriate candidate for accelerating WAS fermentation. In this study, we enriched an incomplete-oxidative sulfate reducing bacteria (io-SRB), combined with SO4∙‾ oxidation (generated by potassium ferrate (PF) and sodium sulfite (Na2SO3)), to boost WAS acetogenesis. Generated sulfate during SO4∙‾ oxidation served as the necessary substrates for io-SRB metabolism. A proof-of-concept based on experimental data for the whole process is presented. Results confirmed that the PF + Na2SO3+ SRB test achieved the maximum SCFAs generation (4261 ± 210 mg COD/L with 60.9 ± 0.5% HAc) over the PF + Na2SO3test without io-SRB mediation (2521 ± 109 mg COD/L with 50.6 ± 0.3% HAc). Particle size analysis and fluorescence spectroscopy indicated that PF + Na2SO3oxidation had positive effects on accelerating soluble organics release. SO4∙‾ was the key radical, playing the most important role, as indicated by electron paramagnetic resonance and radical scavenging analysis. X-ray photoelectron spectroscopy revealed that io-SRB mediation further promoted the transformation of polysaccharides and proteins into carboxylic acids, based on SO4∙‾ oxidation. Moreover, 79% Fe(VI) was reduced to Fe(III), and most S(IV) was converted to SO42−, approximately 40% of which was metabolized by io-SRB consortium. Clearly, SO4∙‾ oxidation and io-SRB stimulation significantly altered the composition of the key microbiome, with fermentative acidogenic bacteria predominating. The possible synergistic relationships among io-SRB, hydrolyzing bacteria and acidogens were revealed by molecular ecological network analysis. This study provides new insights into the improvement of value-added bio-metabolite recovery from SO4∙−-based WAS fermentation.
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影响因子：
6.4
作者：
Zhou, Jizhong;Deng, Ye;Zhi, Xiaoyang
通讯作者：
Zhi, Xiaoyang
影响因子：
15.1
作者：
Feng, Mingbao;Sharma, Virender K.
通讯作者：
Sharma, Virender K.
影响因子：
12.8
作者：
W. Qiao;K. Takayanagi;Qian Li;Mohammad Shofie;Fangshu Gao;R. Dong;Yuyou Li
通讯作者：
W. Qiao;K. Takayanagi;Qian Li;Mohammad Shofie;Fangshu Gao;R. Dong;Yuyou Li
整个玉米秸秆对加强废弃活性污泥酸化有何贡献？
影响因子：
6.3
作者：
Zhou A;Zhang J;Wen K;Liu Z;Wang G;Liu W;Wang A;Yue X
通讯作者：
Yue X
DOI：
10.1039/c8ew00266e
发表时间：
2018-10
期刊：
--
影响因子：
--
作者：
Aijuan Zhou;Zhihong Liu;C. Varrone;Yunbo Luan;Wenzong Liu;Aijie Wang;Xiuping Yue
通讯作者：
Aijuan Zhou;Zhihong Liu;C. Varrone;Yunbo Luan;Wenzong Liu;Aijie Wang;Xiuping Yue