Cloning PIP genes in drought-tolerant vetiver grass and responses of transgenic VzPIP2;1 soybean plants to water stress
Cloning PIP genes in drought-tolerant vetiver grass and responses of transgenic VzPIP2;1 soybean plants to water stress
复制标题
耐旱香根草中 PIP 基因的克隆及转基因 VzPIP2;1 大豆植物对水分胁迫的响应
DOI：
10.1007/s10535-016-0631-5
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发表时间：
2016-12-01
影响因子：
1.5
通讯作者：
Yu, B. J.
中科院分区：
文献类型：
--
作者：
Hu, S. B.;Zhou, Q.;Yu, B. J.
香根草[Vetiveria zizanioides（L.）Nash]表现出全面的非生物胁迫耐受性，这与质膜内在蛋白（PIPs）介导的植物水分关系的良好维持密切相关。从香根草中克隆了两个PIP的开放阅读框架序列（867和873 bp），分别命名为VzPIP 1;1和VzPIP 2;1。绿色荧光蛋白的表达揭示了VzPIP 2;1仅在质膜中的亚细胞定位。 然而，A.发根农杆菌介导的转基因大豆植株通过改善根系水分运输保持了较高的地上部分含水量，并在正常和干旱条件下保持了更有效的光合作用。
Vetiver grass [Vetiveria zizanioides (L.) Nash] displays comprehensive abiotic stress tolerance closely related to fine maintenance of plant water relation mediated by plasma membrane intrinsic proteins (PIPs). Two open reading frame sequences of PIPs (867 and 873 bp) were cloned from vetiver grass and named as VzPIP1;1 and VzPIP2;1, respectively. Expression of green fluorescent protein revealed only subcellular localization of VzPIP2;1 in the plasma membrane. However, A. rhizogenes mediated transgenic soybean plants kept a higher water content in above-ground parts by improving root water transport and kept a more effective photosynthesis under normal and drought conditions.