Quantitative analysis of proteins in the tear fluid of patients with diabetic retinopathy
Quantitative analysis of proteins in the tear fluid of patients with diabetic retinopathy
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DOI：
10.1016/j.jprot.2012.01.019
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发表时间：
2012-04-03
影响因子：
3.3
通讯作者：
Tozser, Jozsef
中科院分区：
文献类型：
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作者：
Csosz, Eva;Boross, Peter;Tozser, Jozsef
糖尿病视网膜病变是发达国家成年人法定失明新病例的主要原因。大约 40% 的糖尿病患者患有糖尿病性视网膜病变，其中 5% 的患者患有视力威胁。由于视力丧失的风险很高，晚期阶段可能没有任何严重症状，有时很难察觉。开发了一种从泪液中检测糖尿病视网膜病变特征生物标志物的非侵入性方法。对来自无视网膜病变、非增殖期和增殖期糖尿病视网膜病变的糖尿病患者的泪液样本进行分析，并将每个样本的蛋白质含量与来自健康志愿者的泪液池的蛋白质含量进行比较。样品用 iTRAQ 四重标记进行标记，并使用 nanoHPLC 耦合 ESI-MS/MS 质谱进行分析。脂质运载蛋白 1、乳转铁蛋白、泪泌素、溶菌酶 C、亲脂蛋白 A 和免疫球蛋白 lambda 链被确定为可能的候选生物标志物，在糖尿病视网膜病变患者的泪液中相对水平显着较高。 (C) 2012 Elsevier B.V. 保留所有权利。
Diabetic retinopathy is the leading cause of new cases of legal blindness among adults in the developed countries. Approximately 40% of all people with diabetes have diabetic retinopathy and 5% of these have sight-threatening form. As the advanced stage, where there is a high risk for vision loss, can develop without any serious symptoms, sometimes it is hard to detect it. A non invasive method to detect biomarkers characteristic for diabetic retinopathy from the tear fluid was developed. Tear samples from diabetic patients with no retinopathy, non proliferative and proliferative stages of diabetic retinopathy were analyzed and the protein content of each sample was compared to the protein content of tear pool from healthy volunteers. The samples were labeled with iTRAQ fourplex labels and were analyzed with nanoHPLC coupled ESI-MS/MS mass spectrometry. The lipocalin 1, lactotransferrin, lacritin, lysozyme C, lipophilin A and immunoglobulin lambda chain were identified as possible biomarker candidates with significantly higher relative levels in the tear of patients with diabetic retinopathy. (C) 2012 Elsevier B.V. All rights reserved.